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I9516

Sigma-Aldrich

2-Propanol

BioReagent, for molecular biology, ≥99.5%

Synonym(s):
sec-Propyl alcohol, IPA, Isopropanol, Isopropyl alcohol
Linear Formula:
(CH3)2CHOH
CAS Number:
Molecular Weight:
60.10
Beilstein:
635639
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.52

grade

for molecular biology

Quality Level

vapor density

2.1 (vs air)

vapor pressure

33 mmHg ( 20 °C)
44 mmHg ( 25 °C)

product line

BioReagent

Assay

≥99.5%

form

liquid

autoignition temp.

750 °F

expl. lim.

2.0-12.7 %, 93 °C

technique(s)

DNA extraction: suitable
RNA extraction: suitable

impurities

≤0.2% water
water

color

colorless

refractive index

n20/D 1.377 (lit.)

bp

82 °C (lit.)

mp

−89.5 °C (lit.)

solubility

water: soluble (completely)

density

0.785 g/mL at 25 °C (lit.)

suitability

suitable for precipitation of nucleic acids

storage temp.

room temp

SMILES string

CC(C)O

InChI

1S/C3H8O/c1-3(2)4/h3-4H,1-2H3

InChI key

KFZMGEQAYNKOFK-UHFFFAOYSA-N

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2-Propanol

grade

for molecular biology

grade

for molecular biology

grade

ACS reagent

grade

ACS reagent

vapor density

2.1 (vs air)

vapor density

2.1 (vs air)

vapor density

2.1 (vs air)

vapor density

2.1 (vs air)

vapor pressure

33 mmHg ( 20 °C), 44 mmHg ( 25 °C)

vapor pressure

33 mmHg ( 20 °C), 44 mmHg ( 25 °C)

vapor pressure

33 mmHg ( 20 °C), 44 mmHg ( 25 °C)

vapor pressure

33 mmHg ( 20 °C), 44 mmHg ( 25 °C)

assay

≥99.5%

assay

≥99.5% (GC)

assay

≥99.5%

assay

≥99.5%

form

liquid

form

liquid

form

liquid

form

liquid

autoignition temp.

750 °F

autoignition temp.

750 °F

autoignition temp.

750 °F

autoignition temp.

750 °F

General description

Isopropanol is a clear, colorless, polar organic solvent commonly used in chemistry and molecular biology laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. This product (2-propanol) is designated as molecular biology grade and is suitable for the precipitation of nucleic acids.

Protocols for the use of isopropanol:

  • DNA precipitation from mammalian tissue, ethidium bromide extraction from DNA samples, and RNA isolation from cells and tissues.
  • Protein crystallization.
  • Isolate retinoids from biological matrices for HPLC and HPLC-MS analysis.

Application

2-Propanol has been used:
  • in DNA and RNA extraction
  • for determination of hydroxyproline levels(The Merck Index, 12th ed)
  • for checking cell viability by MTT assay
  • in microarray hybridization
Suitable for use in DNA precipitation using standard protocols. Also suitable as a solvent for making solutions for molecular biology applications.

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

53.6 °F - closed cup

Flash Point(C)

12.0 °C - closed cup

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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