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Sigma-Aldrich

Phenol – chloroform – isoamyl alcohol mixture

BioUltra, for molecular biology, 25:24:1

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CAS Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

product line

BioUltra

form

liquid

impurities

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

pH

7.2-8.3(H2O-phase, after extraction with H2O, 1:1)

density

1.28 g/mL at 20 °C

absorption

cut-off at 360 nm

storage temp.

2-8°C

SMILES string

ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1

InChI

1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H

InChI key

ZYWFEOZQIUMEGL-UHFFFAOYSA-N

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This Item
25668P455747671
Chloroform – isoamyl alcohol mixture BioUltra, for molecular biology, 49:1, ≥99.5% (chloroform + isoamyl alcohol, GC)

Sigma-Aldrich

25668

Chloroform – isoamyl alcohol mixture

Premium Grade
Phenol solution Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology

Sigma-Aldrich

P4557

Phenol solution

-
Formamide BioUltra, for molecular biology, ≥99.5% (T)

Sigma-Aldrich

47671

Formamide

Premium Grade
form

liquid

form

liquid

form

liquid

form

liquid

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.001% free alkali (as NH3)

impurities

-

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

pH

7.7-8.3(H2O-phase, after extraction with H2O, 1:1)

pH

-

pH

7.7-8.1 (25 °C)

pH

7-9 (25 °C, 1 M in H2O)

density

1.28 g/mL at 20 °C

density

-

density

-

density

1.134 g/mL at 25 °C (lit.)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Phenol-chloroform-isoamyl alcohol mixture (PCI-mixture) is a widely utilized mixture in molecular biology and biochemical research, specifically designed for nucleic acid purification, including DNA and RNA. With its essential composition of 25% phenol, 24% chloroform, and 1% isoamyl alcohol, PCI-mixture plays a pivotal role in separating proteins from nucleic acids during the purification process. Phenol denatures proteins, chloroform creates a distinct layer to trap them, and isoamyl alcohol facilitates the clear separation of these layers, enabling the isolation of purified DNA or RNA in the aqueous phase.
The applications of PCI-mixture are diverse, encompassing RNA isolation, where it efficiently extracts RNA from animal and plant tissues for downstream analyses like reverse transcription and gene expression studies. Further, PCI-mixture also proves instrumental in DNA extraction, enabling efficient recovery for various applications such as DNA minisatellite fingerprinting for genetic identification. As a general purification technique, PCI-mixture ensures high-quality preparations of both DNA and RNA for further research.

Application

Phenol − chloroform − isoamyl alcohol mixture has been used in nucleic acid extraction in bile duct tissue, Drosophila, chromatin and RNA immunoprecipitation in Caenorhabditis elegans and mitochondrial DNA.Further, also in the extraction of DNA for the generation of DNA minisatellite fingerprints

Biochem/physiol Actions

When nucleic acids are extracted with the Phenol-Chloroform-Isoamyl alcohol mixture solution, a clear component separation is achieved. The proteins present in the sample are denatured and removed (collected in the organic phase or in the interphase), while nucleic acids remain in the aqueous phase. The chloroform denatures proteins and facilitates the separation of the aqueous and organic phases, and the isoamyl alcohol reduces foaming during extraction.

Features and Benefits

  • Versatile and adaptable for a wide variety of laboratory and research applications
  • Free from DNase, RNase, NICKase and protease

Physical form

mixture of phenol; chloroform; isoamyl alcohol in the ratio of 25:24:1 (v/v/v); saturated with 100 mM TRIS pH 8.0; contains ~0.1% 8-hydroxyquinoline

related product

Product No.
Description
Pricing

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

174.2 °F

Flash Point(C)

79 °C

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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