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P3803

Sigma-Aldrich

Phenol:Chloroform:Isoamyl Alcohol 25:24:1, Saturated with 10mM Tris, pH 8.0, 1mM EDTA

for molecular biology

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Synonym(s):
DNA extraction
NACRES:
NA.52

grade

for molecular biology

Quality Level

Assay

≥99.0%

form

liquid

pH

6.5-6.9(pH of phenolic phase)

application(s)

agriculture

storage temp.

2-8°C

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This Item
P2069P1944P4682
Phenol solution Saturated with 0.1 M citrate buffer, pH 4.3 ± 0.2, BioReagent, for molecular biology

Sigma-Aldrich

P4682

Phenol solution

assay

≥99.0%

assay

≥99.0%

assay

≥99.0%

assay

-

form

liquid

form

liquid

form

liquid

form

liquid

pH

6.5-6.9(pH of phenolic phase)

pH

6.5-6.9(pH of phenolic phase), 7.8-8.2(pH of phenolic phase after the addition of Equilibration Buffer)

pH

4.3-4.7

pH

4.1-4.5 (25 °C)

application(s)

agriculture

application(s)

agriculture

application(s)

life science and biopharma

application(s)

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

Removal of proteins from nucleic acids can be achieved by extraction with phenol:chloroform: isoamyl alcohol solutions. For crude mixtures of nucleic acids, digestion with a broad range proteolytic enzyme prior to the phenol:chloroform extraction may be beneficial. Although proteins are efficiently denatured by phenol, RNase activity is not completely inhibited. Therefore, a small amount of isoamyl alcohol is added to further ensure the deactivation of RNase activity. Please see Product Information Sheet for the protocol for removal of proteins from nucleic acids.

Warning

If phenol solutions come in contact with skin, burns will occur quickly. It is, therefore, required that the user wear appropriate laboratory clothing including gloves and protective eyewear. In case of direct skin contact, rinse extensively with water and seek medical attention if necessary.

Other Notes

This product has a shelf life of two years.

Recommended products

For DNA extraction, the pH of the phenol phase can be adjusted to 8.0 by equilibration with tris buffer. Sigma offers product P2069 specifically for DNA extractions. This product includes equilibration buffer for the convenience of adjusting the pH.

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Product No.
Description
Pricing

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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25G
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