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C7559

Sigma-Aldrich

Chloroform

≥99%, PCR Reagent, contains amylenes as stabilizer

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Synonym(s):
Methylidyne trichloride, Trichloromethane
Empirical Formula (Hill Notation):
CHCl3
CAS Number:
Molecular Weight:
119.38
Beilstein:
1731042
EC Number:
MDL number:
PubChem Substance ID:

grade

PCR Reagent

Quality Level

vapor density

4.1 (vs air)

vapor pressure

160 mmHg ( 20 °C)

Assay

≥99%

form

liquid

contains

amylenes as stabilizer

packaging

vial of 1.5 mL

technique(s)

PCR: suitable

color

colorless

refractive index

n20/D 1.445 (lit.)

bp

60.5-61.5 °C (lit.)

mp

−63 °C (lit.)

density

1.492 g/mL at 25 °C (lit.)

foreign activity

DNase, RNase, none detected

SMILES string

ClC(Cl)Cl

InChI

1S/CHCl3/c2-1(3)4/h1H

InChI key

HEDRZPFGACZZDS-UHFFFAOYSA-N

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This Item
319988372978472476
Chloroform ≥99%, PCR Reagent, contains amylenes as stabilizer

Sigma-Aldrich

C7559

Chloroform

-
Chloroform contains ethanol as stabilizer, ACS reagent, ≥99.8%

Sigma-Aldrich

319988

Chloroform

Essential Grade
Chloroform anhydrous, contains amylenes as stabilizer, ≥99%

Sigma-Aldrich

372978

Chloroform

-
Chloroform contains amylenes as stabilizer, ACS reagent, ≥99.8%

Sigma-Aldrich

472476

Chloroform

Essential Grade
vapor density

4.1 (vs air)

vapor density

4.1 (vs air)

vapor density

4.1 (vs air)

vapor density

4.1 (vs air)

vapor pressure

160 mmHg ( 20 °C)

vapor pressure

160 mmHg ( 20 °C)

vapor pressure

160 mmHg ( 20 °C)

vapor pressure

160 mmHg ( 20 °C)

assay

≥99%

assay

≥99.8%

assay

≥99%

assay

≥99.8%

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

PCR: suitable

technique(s)

-

technique(s)

-

technique(s)

-

General description

Chloroform is used in pharmaceutical preparations as an anesthetic and as an additive. It is now mainly used as an organic solvent. Chloroform is produced by anthropogenic and natural sources. This abundant halocarbon in the atmosphere is found in ground and surface water sources.

Application

Chloroform has been used in phenol-chloroform extraction to find its ability to purify nucleic acids from blood culture fluids. It has also been used in RNA extraction/isolation and purification.

Pictograms

Skull and crossbonesHealth hazard

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 3

Target Organs

Central nervous system

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 3

Flash Point(F)

does not flash

Flash Point(C)

does not flash

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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25G
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Protocols

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

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