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77619

Supelco

Phenol – chloroform – isoamyl alcohol mixture

BioUltra, for molecular biology, 125:24:1

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CAS Number:
MDL number:
PubChem Substance ID:
NACRES:
ND.02

grade

for molecular biology

Quality Level

product line

BioUltra

form

liquid

impurities

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

pH

4-5(H2O-phase, after extraction with H2O, 1:1)

density

1.12 g/mL at 20 °C

λ

neat

UV absorption

λ: 330 nm Amax: 0.2
λ: 405 nm Amax: 0.1
λ: 510 nm Amax: 0.1

storage temp.

2-8°C

SMILES string

ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1

InChI

1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H

InChI key

ZYWFEOZQIUMEGL-UHFFFAOYSA-N

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This Item
7761725668P4557
Chloroform – isoamyl alcohol mixture BioUltra, for molecular biology, 49:1, ≥99.5% (chloroform + isoamyl alcohol, GC)

Sigma-Aldrich

25668

Chloroform – isoamyl alcohol mixture

Premium Grade
Phenol solution Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, BioReagent, for molecular biology

Sigma-Aldrich

P4557

Phenol solution

-
form

liquid

form

liquid

form

liquid

form

liquid

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected, ≤0.001% free alkali (as NH3)

impurities

-

pH

4-5(H2O-phase, after extraction with H2O, 1:1)

pH

7.2-8.3(H2O-phase, after extraction with H2O, 1:1)

pH

-

pH

7.7-8.1 (25 °C)

density

1.12 g/mL at 20 °C

density

1.28 g/mL at 20 °C

density

-

density

-

UV absorption

λ: 330 nm Amax: 0.2, λ: 405 nm Amax: 0.1, λ: 510 nm Amax: 0.1

UV absorption

-

UV absorption

λ: 260 nm Amax: 0.1, λ: 280 nm Amax: 0.07

UV absorption

-

Physical form

mixture of phenol; chloroform; isoamyl alcohol in the ratio of 125:24:1 (v/v/v); saturated with 2 M NaOAc pH 4

Signal Word

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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