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R1158

Sigma-Aldrich

Ribonuclease Inhibitor

recombinant, expressed in E. coli, 20-40 units/μL

Synonym(s):

RNAse inhibitor

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About This Item

MDL number:
MFCD00132187
UNSPSC Code:
12352200
NACRES:
NA.52

recombinant

expressed in E. coli

Quality Level

300

grade

for molecular biology

form

buffered aqueous solution

mol wt

~50 kDa

concentration

20-40 units/μL

shipped in

dry ice

storage temp.

−20°C

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General description

Ribonuclease inhibitor works to inhibit RNase activity by forming a tight, non-covalent 1:1 complex. It is derived from E. coli which expresses portions of the human placental ribonuclease inhibitor. It inhibits RNases A, B, and C. It will not inhibit RNases H, 1, T1, S1 Nuclease, SP6, T7 RNA Polymerase, T3 RNA Polymerase, AMV Reverse Transcriptase, M-MLV Reverse Transcriptase, or Taq Polymerase. The inhibitor can be removed by phenol extraction or by heating to 65°C for 10 minutes. The pH range for inhibition is pH 5.5–9 (highestinhibition at pH 7–8).

Application

Ribonuclease Inhibitor is suitable for use in useful for in vitro inhibition of ribonucleases, including procedures like:

  • cDNA synthesis from mRNA
  • reverse transcriptase polymerase chain reaction (RT-PCR)
  • in vitro transcription and translation
  • mRNA protection from degradation
  • formaldehyde cross-linked lncRNA purification
  • RNA-fluorescence in situ hybridization (FISH)
Useful for in vitro inhibition of ribonucleases, including procedures like cDNA synthesis, RT-PCR, and in vitro transcription and translation.

Biochem/physiol Actions

Inhibits RNase by forming a tight, non-covalent 1:1 complex.

Components

Ribonuclease Inhibitor is provided as a solution in 20 mM HEPES-KOH( pH 7.6), 50 mM KCl, 8 mM DTT and 50% glycerol.

Unit Definition

One unit will reduce the activity of 5 ng of ribonuclease A by 50% in a cytidine 2′:3′-cyclic monophosphate system.

Physical form

Solution in 20 mM HEPES-KOH, pH 7.6, 50 mM KCl, 8 mM DTT and 50% glycerol.

Other Notes

  • Denaturing conditions (i.e., urea or temperatures ≥50 °C) should be avoided as they may cause a release of active ribonuclease from the complex.
  • This product is for R&D use only, not for drug, household, or other uses.

related product

Product No.
Description
Pricing

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Antisense overlapping long non-coding RNA regulates coding arachidonate 12-lipoxygenase gene by translational interference
Sabbir MG, et al.
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1866(10), 158987-158987 (2021)
Lintao Li et al.
Cell cycle (Georgetown, Tex.), 22(4), 476-493 (2022-11-13)
Whether long non-coding RNA Mir-99a-Let-7c Cluster Host Gene (LncRNA MIR99AHG) is involved in osteoporosis (OP) remains vague, so we hereby center on its implication. Old C57BL/6J mice were injected with the silencing lentivirus of MIR99AHG and subjected to microCT analysis
A fine-needle aspiration-based protein signature discriminates benign from malignant breast lesions
Franzen B, et al.
Molecular Oncology, 12(9), 1415-1428 (2018)
Nathan H Vande Burgt et al.
Experimental parasitology, 187, 30-36 (2018-03-09)
The water-borne protozoan parasite Cryptosporidium parvum forms oocysts that can persist for long periods of time in the environment, even though the sporozoites inside the oocysts may no longer be viable, making it difficult to assess the associated risk of
Comparison of in vitro viability methods for Cryptosporidium oocysts
Burgt NHV, et al.
Experimental Parasitology, 187, 30-36 (2018)
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