Skip to Content
MilliporeSigma
All Photos(2)

Key Documents

03-0145-00

Millipore

SMC® Amyloid Beta 1-40 High Sensitivity Immunoassay Kit

Synonym(s):

Abeta 1-40 immunoassay kit, Abeta 40 Single Molecule Counting kit, Abeta 40 immunoassay kit, Abeta 40 ultra-sensitive immunoassay

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.84

manufacturer/tradename

SMC®

Quality Level

assay range

sensitivity: 5.86 pg/mL
(lower limit of quantification)

inter-assay cv: <10%
intra-assay cv: <10%
(Intra-assay CV n=20; Inter-assay n=5)

technique(s)

ELISA: suitable
single molecule counting: suitable

General description

Amyloid Beta refers to the short peptides that are released into circulation following the proteolysis of the transmembrane protein amyloid precursor protein (APP), whose function is unclear but thought to be involved in neuronal development and function. The most common peptides generated from APP cleavage are Aβ1-40 and Aβ1-42, with the latter isoform implicated in the formation of toxic oligomers, fibrils, and plaques in the diseased brain. Reduced levels of soluble Aβ1-42 in the cerebrospinal fluid (CSF) are associated with Alzheimer’s disease (AD), leading to the adoption of this peptide as an AD biomarker. The presence of AB40, AB42 in patient CSF and plasma changes as an individual ages, and increases in AB42 levels, and more importantly, the AB42/AB40 ratio, are recognized as correlating with dementia and the onset of Alzheimer’s.
The SMC® Amyloid Beta 1-40 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna and SMCxPRO immunoassay systems detect analytes using Single Molecule Counting (SMC) technology.
The sensitivity of the Single Molecule Counting (SMC®) technology gives the SMC Amyloid Beta 1-40 (Aβ40) High Sensitivity Immunoassay Kit unparalleled performance in the quantification of Aβ40 in human, mouse and rat cerebrospinal fluid (CSF) and plasma.

Application

Research Category
Neuroscience

Metabolism
Research Sub Category
Inflammation & Autoimmune Mechanisms

Metabolism

Obesity
The sensitivity of the Single Molecule Counting (SMC®) technology gives the SMC Amyloid Beta 1-40 (Aβ40) High Sensitivity Immunoassay Kit unparalleled performance in the quantification of Aβ40 in human, mouse and rat cerebrospinal fluid (CSF) and plasma.

Components

Assay Buffer

SMC Amyloid Beta 1-40 Coated Beads

SMC Amyloid Beta Standard Diluent

SMC Amyloid Beta Detection Antibody

SMC Amyloid Beta 1-40 Standard

10X Wash Buffer

Buffer D

Elution Buffer B

Specifications

Minimum of 5 CSF samples spiked with average recovery of 102% with a range of 95-106% and 5 plasma samples spiked with average recovery of 97% with a range of 92-100%.

Legal Information

SMC is a registered trademark of Singulex

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Irrit. 2 - Repr. 1B

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Chia-Liang Tsai et al.
Journal of clinical medicine, 8(10) (2019-10-09)
Nondemented people with a family history of Alzheimer's disease (ADFH) and the ApoE-4 allele have been demonstrated to show a trend for a higher probability of cognitive decline and aberrant levels of cognitive-related biomarkers. However, the potential interactive effects on

Protocols

To determine protein content, the Warburg-Christian method refers to measuring protein samples at 280 nm using a spectrophotometer.

Related Content

MILLIPLEX® multiplex immunoassays detect Alzheimer's biomarkers in neuroscience research using high sensitivity technology.

Protein quantification methods, reagents, and immunoassay technology for accurately measuring the protein concentrations in a variety of samples.

Neuroscience biomarker research expands with high-sensitivity immunoassays for low-abundant biomarkers.

Explore high-sensitivity SMC® neurology immunoassays that precisely measure low levels of neurology protein analytes, such as NF-L or Tau proteins.

See All

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service