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04-263

Sigma-Aldrich

Anti-Ubiquitinylated proteins Antibody, clone FK2

clone FK2, Upstate®, from mouse

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eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

FK2, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Upstate®

technique(s)

ELISA: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

shipped in

dry ice

target post-translational modification

unmodified

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This Item
04-262MABS451ABS2206
antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified antibody

clone

FK2, monoclonal

clone

FK1, monoclonal

clone

LUB9, monoclonal

clone

polyclonal

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

ELISA: suitable, immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

western blot: suitable

technique(s)

affinity binding assay: suitable, western blot: suitable

isotype

IgG1

isotype

IgM

isotype

IgG2bκ

isotype

IgG

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General description

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.

Specificity

Recognizes mono- and poly ubiquitinylated proteins, but not free ubiquitin.

Immunogen

Poly-ubiquitinylated-lysozyme.

Application

Research Category
Protein Trafficking
Research Sub Category
Ubiquitin & Ubiquitin Metabolism
This Anti-Ubiquitinylated proteins Antibody, clone FK2 has been published and validated for use in ELISA, Immunofluorescence (IF), Immunoprecipitation (IP) and Western Blotting (WB) for the detection of Ubiquitinylated proteins.

Physical form

100 µL of purified immunoglobulin in PBS containing 0.1% sodium azide. Dilute to working strength with phosphate buffered saline pH 7.2-7.4.
Format: Purified

Storage and Stability

Stable for 1 year at -20°C from date of shipment for up to 12 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Production and characterization of monoclonal antibodies specific to multi-ubiquitin chains of polyubiquitinated proteins.
Fujimuro, M, et al.
Febs Letters, 349, 173-180 (1994)
Autophagy promotes T-cell survival through degradation of proteins of the cell death machinery.
Kovacs, JR; Li, C; Yang, Q; Li, G; Garcia, IG; Ju, S; Roodman, DG; Windle, JJ; Zhang, X; Lu, B
Cell Death and Differentiation null
Dynamics of ubiquitin conjugation during heat-shock response revealed by using a monoclonal antibody specific to multi-ubiquitin chains
Fujimuro, M, et al
European Journal of Biochemistry, 249, 427-433 (1997)
Sungsu Lim et al.
Nature communications, 4, 2641-2641 (2013-10-19)
Gene expression is an intricate process tightly linked from gene activation to the nuclear export of mRNA. Recent studies have indicated that the proteasome is essential for gene expression regulation. The proteasome regulatory particle binds to the SAGA complex and
Tamar Listovsky et al.
The Journal of cell biology, 203(1), 87-100 (2013-10-09)
The switch from activation of the anaphase-promoting complex/cyclosome (APC/C) by CDC20 to CDH1 during anaphase is crucial for accurate mitosis. APC/C(CDC20) ubiquitinates a limited set of substrates for subsequent degradation, including Cyclin B1 and Securin, whereas APC/C(CDH1) has a broader

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