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07-375

Sigma-Aldrich

Anti-Ubiquitin Antibody

Upstate®, from rabbit

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eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

form

(Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide with 30% glycerol.)

mol wt

(~8 kDa observed. Uncharacterized bands may be observed in some lysate(s).)

species reactivity

bovine, human

species reactivity (predicted by homology)

vertebrates, (Predicted to react with Vertebrates.)

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... UBAP2(55833)

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This Item
07-2130MABS486U5379
vibrant-m

07-375

Anti-Ubiquitin Antibody

vibrant-m

07-2130

Anti-Ubiquitin Antibody

species reactivity

bovine, human

species reactivity

rat, human, mouse

species reactivity

human

species reactivity

prokaryotes, eukaryotes

antibody form

purified immunoglobulin

antibody form

-

antibody form

purified antibody

antibody form

whole antiserum

clone

polyclonal

clone

polyclonal

clone

2G7B8, monoclonal

clone

polyclonal

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

shipped in

-

mol wt

(~8 kDa observed. Uncharacterized bands may be observed in some lysate(s).)

mol wt

-

mol wt

-

mol wt

antigen 8.6 kDa

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General description

Anti-Ubiquitin, Cat. No. 07-375, is a rabbit polyclonal antibody that detects ubiquitin and ubiquitinylated proteins and is tested for use in Western Blotting.

Specificity

This rabbit polyclonal antibody specifically detects Ubiquitin.

Immunogen

Full-length ubiquitin is isolated from bovine erythrocytes.

Application

Anti-Ubiquitin, Cat. No. 07-375, is a rabbit polyclonal antibody that detects ubiquitin and ubiquitinylated proteins and is tested for use in Western Blotting.
Research Category
Signaling
Research Sub Category
Ubiquitin & Ubiquitin Metabolism

Quality

routinely evaluated by immunoblot from bovine erythrocytes, and ubiquitylated proteins in acid extracts from HeLa cells

Target description

Ubiquitin (Ub) is initially produced as a 229 amino acids Polyubiquitin-B (UniProt: P0CG47) precursor protein encoded by the UBB gene (Gene ID: 7314) or a 685 amino acids Polyubiquitin-C precursor protein (UniProt: P0CG48) encoded by the UBC gene (Gene ID: 7316) in human. Ub exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different lysine residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator methionine (Met) of the ubiquitin (linear polyubiquitin chains). Ub is linked covalently via its carboxyl terminus (Gly76) to lysine residues in target proteins. In a given target lysine residue can be linked to one single Ub molecule (monoubiquitylated) or to a chain of Ub molecules (polyubiquitylated). In a polyUb chain, Ub molecules can be linked through one of the seven lysine residues (K6, K11, K27, K29, K33, K48, and K63). Polyubiquitin chains, when attached to a target protein, have different functions depending on the lysine residue of the ubiquitin that is linked. For example, lysine 6-linked may be involved in DNA repair; lysine 11-linked is involved in endoplasmic reticulum-associated degradation (ERAD) and in cell-cycle regulation, and lysine 29-linked is involved in lysosomal degradation. Lysine 48-linked chains mark proteins for proteasomal degradation, while lysine 63-linked chains are involved in endocytosis, DNA-damage responses, and in signaling leading to activation of the transcription factor NF- B. Ubiquitin undergoes phosphorylation at the serine 57 by a ubiquitin kinase and this phosphorylation is an important modifier of ubiquitin function, particularly in response to proteotoxic stress. This phosphorylation may also be a deciding factor whether ubiquitin is recycled or degraded during multi-vesicular body sorting on endosomes. (Ref.: Hepowit, NL., et al (2020). eLife 9; e58155; Lee, S., et al. (2017) eLife. 6; e29176).

Linkage

Replaces: 04-454

Physical form

Format: Purified
Protein A Purified immunoglobulin in PBS, 0.1% sodium azide, and 30% glycerol.
Protein A purified

Storage and Stability

Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
All tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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Vertebrate DNA crosslink repair excises toxic replication-blocking DNA crosslinks. Numerous factors involved in crosslink repair have been identified, and mutations in their corresponding genes cause Fanconi anemia (FA). A key step in crosslink repair is monoubiquitination of the FANCD2-FANCI heterodimer
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