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1.04148

Millipore

Singlepath® Listeria monocytogenes

suitable for lateral flow assay, for Listeria monocytogenes, input: food(s)

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Synonym(s):
Rapid L. Mono Food Test

form

solid

Quality Level

shelf life

15 mo.

packaging

pack of 25 strips

manufacturer/tradename

Singlepath®

technique(s)

lateral flow assay: suitable

input

sample type food(s)

application(s)

food and beverages
pathogen testing

storage temp.

2-8°C

suitability

Listeria monocytogenes

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suitability

Listeria monocytogenes

suitability

Escherichia coli (O157), enterovirulent E. coli (EEC)

suitability

Campylobacter spp.

suitability

Citrobacter spp., Salmonella spp.

input

sample type food(s)

input

food(s)

input

sample type food(s)

input

food(s)

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

application(s)

food and beverages
pathogen testing

application(s)

food and beverages
pathogen testing

application(s)

food and beverages
pathogen testing

application(s)

food and beverages
pathogen testing

technique(s)

lateral flow assay: suitable

technique(s)

lateral flow assay: suitable, microbe id | immunological: suitable

technique(s)

lateral flow assay: suitable, microbe id | immunological: suitable

technique(s)

lateral flow assay: suitable, microbe id | immunological: suitable

General description

Singlepath® Listeria monocytogenes is a rapid diagnostic test, designed to detect Listeria monocytogenes bacteria in various food samples. The Singlepath® Listeria monocytogenes is an immunochromatographic rapid test based on gold-labeled antibodies to detect the presence of a specific antigen, in this case, the L. monocytogenes bacteria. This lateral flow test is based on the immune flow principle and is a convenient and simple-to-use, one-step device. The test includes a nitrocellulose membrane strip that is coated with antibodies specific to L. monocytogenes antigens. When the sample is applied to the test strip, any L. monocytogenes antigens present will bind to the antibodies on the strip, resulting in the development of a visible test line. The test takes approximately 20 minutes to complete, and the results are interpreted based on the presence or absence of the test line. The lower detection limit can be considered as 5x106 bacteria/ml or one colony on an agar plate, depending on the serotype. No additional equipment expenditure is needed.

Application

Singlepath® Listeria monocytogenes can be used in industrial microbiology, to test a wide range of foods for Listeria monocytogenes using Listeria selective agars (e.g. PALCAM, Oxford, Chromoplate Listeria, Chromocult Listeria, etc.). This test can also be used for confirmation of suspect Listeria monocytogenes colonies on these agars.

Features and Benefits

  • Rapid and reliable method
  • High sensitivity and specificity compared to conventional culture methods
  • Can be performed without the need for specialized equipment or personnel.

Linkage

Replaces: 1.04148.0002

Analysis Note

Performance test Antigen (Listeria monocytogenes Sv 1/2a EGD): passes test
Performance test Antigen (Listeria monocytogenes Sv 1/2c): passes test
Performance test Antigen (Listeria innocua ATCC 33090 (WDCM 00017)): passes test
Performance test Antigen (Listeria ivanovii ATCC 19119 (WDCM 00018)): passes test
Performance test Antigen (Escherichia coli ATCC 25922 (WDCM 00013)): passes test
Performance test Culture (Listeria monocytogenes Sv 1/2a EGD): passes test
Performance test Culture (Listeria innocua ATCC 33090 (WDCM 00017)): passes test
Performance test Culture (Listeria ivanovii ATCC 19119 (WDCM 00018)): passes test

Legal Information

SINGLEPATH is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids


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Related Content

Detect pathogens rapidly using our lateral flow immunological tests which act as mini-laboratories for identifying the presence of specific pathogens.

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