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407652

Sigma-Aldrich

Interleukin-6, Human, Recombinant, E. coli

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Synonym(s):
BSF-2, IFN-beta-2, IFNB2, rhIL-6

assay

≥95% (SDS-PAGE)

Quality Level

form

lyophilized

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

technique(s)

cell culture | mammalian: suitable

impurities

≤1.0 EU/μg Endotoxin (EU/μg IL-6)

NCBI accession no.

UniProt accession no.

shipped in

ambient

storage temp.

−70°C

Gene Information

human ... IL6(3569)

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407654407711407700
technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

-

form

lyophilized

form

lyophilized

form

lyophilized

form

lyophilized

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage condition

OK to freeze

storage condition

OK to freeze

storage condition

OK to freeze

storage condition

OK to freeze

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

Research area: Immunology and Cytokines

Human sequence IL-6 is active in murine cells.1 µg is equivalent to 7.0 x 103 WHO units. 8-20 pg/ml as measured in a cell proliferation assay with a factor-dependent murine plasmacytoma cell line, B9.

application

Interleukin-6has been used:
  • to study IL-6 effects on lung cancer-derived mesenchymal stromalcells (MSCs) and to test the ability of statins to block these effects.
  • toinvestigate the role of IL-6 in the development of coronary artery spasm (CAS).
  • to study the effects of IL-6 on the bioactivity of therapeutic interferon (IFN)variants.

Biochem/physiol Actions

Interleukin-6 (IL-6) is a prototypic pleiotropic, pro-inflammatory cytokine that is expressed in response to injury, inflammation, and infection.(5) IL-6 triggers the activation of three distinct pathways: signal transducer and activator of transcription 3 (STAT-3), extracellular signal-regulated kinase 1 and 2 (Erk1/2), and protein kinase b/protein kinase b-alpha (Akt). Consequently, this activation leads to the upregulation of numerous genes that impact essential cellular processes including survival, proliferation, differentiation, osteogenesis/osteolysis, angiogenesis, and immune modulation. It stimulates the production of acute-phase proteins by hepatocytes. IL-6 is also known to induce skeletal muscle protein breakdown. Induces growth and differentiation of B cells, T cells, and hepatocytes. Stimulates growth and inhibits constitutive, protein synthesis-independent apoptosis of murine B cell hybridoma 7TD1. IL-6 is also considered as a potential marker of adipocyte differentiation. Interleukin-6 is a protein produced by a variety of cell types including T-lymphocytes, monocytes, diploid fibroblasts, hepatocytes, cardiac myxomas, certain cervical cancer cells, bladder cell carcinomas, vascular endothelial cells, and human myelomas. It has a variety of biological effects and does not show a high degree of species specificity.

Warning

Toxicity: Standard Handling (A)

Physical form

Carrier free.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 3 months at -70°C. Avoid freeze/thaw cycles of solutions.
Reconstitute to 0.1-1.0 mg/ml in 100 mM acetic acid.

Other Notes

Vicennati, V., et al. 2002. Int. J. Obes. Relat. Metab. Disord.26, 9.
Vallieres, L., and Rivest, S. 1999. Endocrinology140, 3890.
Goodman, M.N. 1994. Proc. Soc. Exp. Biol. Med. 205, 182.
Liu, J., et al. 1994. Cell Immunol.155, 229.
Van Snick, J. 1990. Annu. Rev. Immunol.8, 253.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Louise Larocque et al.
Journal of biomedicine & biotechnology, 2011, 174615-174615 (2011-03-16)
The traditional antiviral assays for the determination of interferon potency are reported to have considerable variability between and within assays. Although several reporter gene assays based on interferon-inducible promoter activities have been reported, data from comprehensive validation studies are lacking
Yen-Kuang Lin et al.
Oxidative medicine and cellular longevity, 2022, 9964689-9964689 (2022-02-01)
Apolipoprotein (a)/lipoprotein(a) (Lp(a)), a major carrier of oxidized phospholipids, and α7-nicotinic acetylcholine receptor (α7-nAChR) may play an important role in the development of coronary artery spasm (CAS). In CAS, the association between Lp(a) and the α7-nAChR-modulated inflammatory macrophage polarization and
Johnathan Ho et al.
PLoS biology, 14(10), e2000117-e2000117 (2016-10-26)
STAT2 is the quintessential transcription factor for type 1 interferons (IFNs), where it functions as a heterodimer with STAT1. However, the human and murine STAT2-deficient phenotypes suggest important additional and currently unidentified type 1 IFN-independent activities. Here, we show that
Sabine Galland et al.
Cancer letters, 484, 50-64 (2020-05-19)
Solid tumor growth triggers a dynamic host response, which recapitulates wound healing and defines the tumor microenvironment (TME). In addition to the action of the tumor cells themselves, the TME is maintained by a myriad of immune and stromal cell-derived

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