MilliporeSigma
All Photos(9)

Documents

AB152

Sigma-Aldrich

Anti-Tyrosine Hydroxylase Antibody

Chemicon®, from rabbit

Sign Into View Organizational & Contract Pricing

Synonym(s):
Tyrosine 3-hydroxylase, Tyrosine 3-monooxygenase, tyrosine hydroxylase, TH
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mollusk, feline, ferret, Aplysia (marine mollusc), rat, squid, Drosophila, mouse

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... TH(7054)
rat ... Th(25085)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
AB5935AB1542AB9983
vibrant-m

AB152

Anti-Tyrosine Hydroxylase Antibody

vibrant-m

AB1542

Anti-Tyrosine Hydroxylase Antibody

vibrant-m

AB9983

Anti-Tyrosine Hydroxylase Antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

technique(s)

ELISA: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable, immunofluorescence: suitable, immunoprecipitation (IP): suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

wet ice

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Tyrosine hydroxylase (also know as Tyrosine 3-hydroxylase and Tyrosine 3-monooxygenase) plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

Specificity

It is expected that the antibody will react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin embedded human tissue.
Tyrosine hydroxylase. By western blot, AB152 selective labels a single band at approximately 62kDa (reduced) corresponding to Tyrosine Hydroxylase. It is expected that the antibody will react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin embedded human tissue.

Immunogen

Denatured tyrosine hydroxylase from rat pheochromocytoma (denatured by sodium dodecyl sulfate).

Application

Anti-Tyrosine Hydroxylase Antibody detects level of TH and has been published and validated for use in ELISA, IF, IH, IH(P), IP and WB.
Immunohistochemistry:
A previous lot of this antibody was uesd at 1:100-1:1,000 dilution, 4°C, for 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase.

Immunofluorescence:
A previous lot of this antibody was used at 1:100-1:1,000 dilution.

Immunoprecipitation:
A previous lot of this antibody was used on Immunoprecipitation.

ELISA:
A previous lot of this antibody was used in ELISA.

Optimal dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Neuronal & Glial Markers

Quality

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of PC12 lysates.

Target description

62 kDa

Physical form

Affinity purified
Purified rabbit polyclonal in buffer containing liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl (pH 7.5), 100 ug/mL BSA and 50% glycerol.

Storage and Stability

Maintain frozen at -20ºC to -70ºC in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.

Analysis Note

Control
POSITIVE CONTROL: Brain (corpus striatum, sympathetic nerve terminals) and adrenal glands.
NEGATATIVE CONTROL: Liver.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Writing memories with light-addressable reinforcement circuitry.
Claridge-Chang, A; Roorda, RD; Vrontou, E; Sjulson, L; Li, H; Hirsh, J; Miesenbock, G
Cell null
Axon guidance of mouse olfactory sensory neurons by odorant receptors and the beta2 adrenergic receptor.
Feinstein, P; Bozza, T; Rodriguez, I; Vassalli, A; Mombaerts, P
Cell null
Morphologic pattern of the intrinsic ganglionated nerve plexus in mouse heart.
Rysevaite, K; Saburkina, I; Pauziene, N; Noujaim, SF; Jalife, J; Pauza, DH
Heart Rhythm : the Official Journal of the Heart Rhythm Society null
Bret C Mobley et al.
PloS one, 10(7), e0133897-e0133897 (2015-07-30)
Neuroblastoma is a pediatric malignancy of the sympathetic ganglia and adrenal glands, hypothesized to originate from progenitors of the developing sympathetic nervous system. Amplification of the MYCN oncogene is a genetic marker of risk in this disease. Understanding the impact
Alfredo Rodríguez-Cruz et al.
IBRO reports, 8, 28-35 (2020-01-08)
Parkinson´s disease is the most important neuromotor pathology due to the prominent loss of dopaminergic neurons in the substantia nigra pars compacta. There is an inherent deficiency of dopamine in Parkinson´s disease, which is aggravated when neuroinflammatory processes are present.

Articles

Leverage the inherent immune ability of an animal to generate antibodies that bind to specific molecules.

Explore the basics of working with antibodies including technical information on structure, classes, and normal immunoglobulin ranges.

Uses for immunofluorescence (IF)—where an antibody conjugated to a molecule that fluoresces when excited by lasers— include protein localization, confirmation of post-translational modification or activation, and proximity to/complexing with other proteins.

Antibodies combine with specific antigens to generate an exclusive antibody-antigen complex. Learn about the nature of this bond and its use as a molecular tag for research.

See All

Protocols

Tips and troubleshooting for FFPE and frozen tissue immunohistochemistry (IHC) protocols using both brightfield analysis of chromogenic detection and fluorescent microscopy.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service