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Key Documents

AB5620

Sigma-Aldrich

Anti-Neurogranin Antibody

Chemicon®, from rabbit

Synonym(s):

Anti-RC3

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

species reactivity

chicken, zebrafish, human, bovine, mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... NRGN(4900)

Specificity

Recognizes Neurogranin.

Immunogen

Recombinant rat Neurogranin (complete sequence).

Application

Immunoblot: >1:500-1:000

Immunohistochemistry: >1:1,000 on 4% paraformaldehyde / PBS fixed or 3% Paraformaldehyde / 0.1% Gluteraldehyde / PBS fixed tissue sections.

Immunocytochemistry: >1:1,000.

Immunoprecipitation: >1:100

ELISA: >1:2,000

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Signaling Neuroscience
This Anti-Neurogranin Antibody is validated for use in ELISA, IC, IH, IP, WB for the detection of Neurogranin.

Target description

7.5 kDa

Physical form

Ammonium Sulfate Precipitation
Format: Purified
Purified immunoglobulin. Purified by ammonium sulfate precipitation. Liquid in PBS with 0.02% sodium azide as a preservative.

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Sandra L Wilson et al.
Cerebellum (London, England), 10(3), 356-372 (2011-03-25)
The cerebellum is a highly organized structure partitioned into lobules along the anterior-posterior (A-P) axis and into striped molecular domains along the medial-lateral (M-L) axis. The Engrailed (En) homeobox genes are required for patterning the morphological and molecular domains along
Elisabetta Cesana et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 33(30), 12430-12446 (2013-07-26)
The function of inhibitory interneurons within brain microcircuits depends critically on the nature and properties of their excitatory synaptic drive. Golgi cells (GoCs) of the cerebellum inhibit cerebellar granule cells (GrCs) and are driven both by feedforward mossy fiber (mf)
Neurogranin alters the structure and calcium binding properties of calmodulin.
Hoffman, L; Chandrasekar, A; Wang, X; Putkey, JA; Waxham, MN
The Journal of Biological Chemistry null
Roy V Sillitoe et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 28(11), 2820-2826 (2008-03-14)
Despite the general uniformity in cellular composition of the adult cerebellar cortex, there is a complex underlying pattern of parasagittal stripes of Purkinje cells with characteristic molecular phenotypes and patterns of connectivity. It is not known whether interneuron processes are
Joshua J White et al.
Frontiers in neuroanatomy, 7, 9-9 (2013-05-16)
The cerebellum is organized into parasagittal zones that control sensory-motor behavior. Although the architecture of adult zones is well understood, very little is known about how zones emerge during development. Understanding the process of zone formation is an essential step

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