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ABC338

Sigma-Aldrich

Anti-IRGM Antibody

from rabbit, purified by affinity chromatography

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Synonym(s):
IRGM Antibody, Immunity related GTPase family, IFL1, IRGM1, LRG47, LRG-47
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... IRGM(345611)

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This Item
HPA043254HPA020266HPA050338
Anti-IRGM Antibody from rabbit, purified by affinity chromatography

ABC338

Anti-IRGM Antibody

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

UniProt accession no.

A1A4Y4

UniProt accession no.

Q8WZA9

UniProt accession no.

Q8NHV1

UniProt accession no.

Q8WZA9

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

Gene Information

human ... IRGM(345611)

Gene Information

human ... IRGQ(126298)

Gene Information

human ... GIMAP7(168537)

Gene Information

human ... IRGQ(126298)

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General description

Immunity-related GTPase family M protein (IRGM), also known as Interferon-inducible protein 1 (IFI1) and LPS-stimulated RAW 264.7 macrophage protein 47 homolog (LRG-47), is a member of the interferon-inducible GTPase family. It induces autophagy and generates large autolysosomal organelles as a mechanism for the elimination of intracellular Mycobacterium tuberculosis. IRGM is expressed in colon, small bowel, and peripheral blood leukocytes. Mutations in IRGM have been assoicated with inflammatory bowel disease type 19 which is subdivided into Crohn′s disease and ulcerative colitis phenotypes.

Specificity

This antibody recognizes the intermediate domain of IRGM.

Immunogen

KLH-conjugated linear peptide corresponding to the intermediate domain of human IRGM.

Application

Detect Immunity-related GTPase family M protein using this rabbit polyclonal antibody, Anti-IRGM Antibody validated for use in western blotting, IHC (Paraffin) & Immunofluorescence.
Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected IRGM in human brain tissue.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected IRGM in human brain cells.

Quality

Evaluated by Western Blotting in human brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected IRGM in 15 µg of human brain tissue lysate.

Target description

~31 kDa observed. Uncharacterized bands may appear in some lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Hironori Bando et al.
mBio, 9(5) (2018-10-12)
Although Toxoplasma virulence mechanisms targeting gamma interferon (IFN-γ)-induced cell-autonomous antiparasitic immunity have been extensively characterized in mice, the virulence mechanisms in humans remain uncertain, partly because cell-autonomous immune responses against Toxoplasma differ markedly between mice and humans. Despite the identification

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