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CC050

Sigma-Aldrich

Human Collagen Type I

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eCl@ss:
32160405
NACRES:
NA.75

biological source

human

Quality Level

assay

>90% (collagen type I, SDS-PAGE)

form

liquid

manufacturer/tradename

Chemicon®

concentration

1 mg/mL

technique(s)

cell culture | mammalian: suitable

impurities

<1% collagen type II,IV-VI & non-collagen proteins.
<10% collagen type III

input

sample type pancreatic stem cell(s)
sample type mesenchymal stem cell(s)
sample type epithelial cells
sample type induced pluripotent stem cell(s)
sample type neural stem cell(s)
sample type: human embryonic stem cell(s)
sample type hematopoietic stem cell(s)

solubility

water: soluble at 20 °C

NCBI accession no.

UniProt accession no.

binding specificity

Peptide Source: Elastin

Peptide Source: Fibronectin

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... COL1A1(1277)

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1 of 4

This Item
C6745CC052CC054
vibrant-m

CC050

Human Collagen Type I

vibrant-m

CC052

Human Collagen Type II

vibrant-m

CC054

Human Collagen Type III

assay

>90% (collagen type I, SDS-PAGE)

assay

-

assay

90% (Human collagen type II)

assay

90% (Human collagen type III, SDS-PAGE)

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

biological source

human

biological source

human cell culture

biological source

human

biological source

human

concentration

1 mg/mL

concentration

0.3 mg/mL

concentration

1 mg/mL

concentration

1 mg/mL

Gene Information

human ... COL1A1(1277)

Gene Information

human ... COL4A1(1282)

Gene Information

human ... COL2A1(1280)

Gene Information

human ... COL3A1(1281)

General description

Human type I collagen is purified by serial salt precipitations, alcohol precipitation and DEAE chromatography of a pepsin extraction of human placenta. Collagen is a major structural protein found in connective tissues such as skin, tendon, cartilage, ligaments, bone, the part of the eyeball that is white (sclera), and the spaces between cells and tissues called the extracellular matrix. It imparts structure and strength to the connective tissues. The gene for collagen type I alpha 1 (COL1A1) is mapped to human chromosome 17q21.33.Type I collagen is initially produced as procollagen in cells. This protein consists of two pro-alpha1(I) protein strands combined with a pro-alpha2(I) procollagen strand that form a triple-stranded rope-like structure. While in the cell, enzymes modify certain amino acids in the protein (lysine and proline) by adding chemical groups that are necessary for the three strands to form stable molecules and make connections (cross-links) between chains. Other enzymes add sugars to the protein. Now complete, the triple-stranded type I procollagen molecule leaves the cell and is processed by enzymes that clip small segments off both ends. The procollagen molecules arrange themselves into long, thin fibrils outside of the cell. The fibrils come together in side-by-side groups to form collagen fibers. Cross-linking between molecules in fibrils produces a very stable protein structure, which contributes to collagen tissue strengthening function.

Application

Human Collagen Type I has been used:
  • as a control in the 2B4 nuclear factor of activated T-cells (NFAT)–GFP reporter cell assay, where its interaction with reporter cells can be evaluated for nuclear factor of activated T-cells (NFAT) promoter-driven GFP expression
  • for coating glass slides in dynamic binding assays to create a substrate for the specific binding and study of platelets and conjugates in flow channels
  • as a protein standard in histological analysis of lung tissue samples, providing a reference for the composition and characterization of extracellular matrix (ECM) components

Biochem/physiol Actions

Mutations in the collagen type I alpha 1 (COL1A1) gene are associated with osteogenesis imperfecta types I–IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.

Physical form

Purified protein. Liquid containing 0.5 M Acetic acid, pH 2.5. Can be diluted in PBS for applications.

Analysis Note

Purity was controlled by SDS-PAGE and reaction with anti-collagen type-specific antibodies

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

Extracellular matrix proteins such as laminin, collagen, and fibronectin can be used as cell attachment substrates in cell culture.

Protocols

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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