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IPFL07810

Immobilon®-FL PVDF Membrane

1 roll, 7 x 8.4cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane with low background fluorescence for Western blotting. Compatible with visible and infrared fluorescent probes.

Synonym(s):

Western blotting membrane, blotting membrane, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red
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Product Name

Immobilon®-FL PVDF Transfer Membrane, 0.45 μm pore size, 7 X 8.4 cm sheets

Quality Segment

400

material

PVDF membrane, plain filter, white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

filter L × W

7 cm × 8.4 cm

pore size

0.45 μm pore size

capacity

155 μg/cm2 adsorption capacity (insulin), 205 μg/cm2 adsorption capacity (BSA), 300 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Coomassie brilliant blue, for use with Ponceau-S red

detection method

chemiluminescent, colorimetric, fluorometric

shipped in

ambient

General description

The Immobilon®-FL transfer membrane is a polyvinylidene fluoride (PVDF) microporous membrane for binding proteins transferred from a variety of gel matrices. The Immobilon®-FL membrane has a nominal pore size of 0.45 μm and is recommended for blotting proteins with molecular weights greater than 20 kilodaltons (kDa). The membrane exhibits very low autofluorescence across a wide range of excitation/emission wavelengths. This property makes it ideal for blotting applications involving fluorescence-based immunodetection.
Filter Code: IPFL

Application

Immobilon®-FL Transfer Membrane has been used in western blotting.

Features and Benefits

Immobilon®-FL Transfer Membrane:
  • Designed for optimal fluorescent immunodetection application
  • Extremely low background improves the sensitivity of all fluorescence based immunodetection
  • Compatible with all commonly used fluorescent probes across a wide range of excitation/emission wavelengths
  • For standard chemiluminescent or chromogenic detection

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Related Content

Immobilon®-P Transfer Membrane User Guide
Protein Blotting Handbook: 6th Edition

Protein Blotting Survival Handbook: Tips and Tricks

Antibody recovery and reuse in the SNAP i.d.®2.0 immunodetection system

Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.

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Global Trade Item Number

SKUGTIN
IPFL0781004053252372605