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Key Documents

MAB13431

Sigma-Aldrich

Anti-MMP-2 Antibody, pro and active form, clone A-Gel VC2

clone A-Gel VC2, Chemicon®, from mouse

Synonym(s):

Gelatinase A, 72 kDa Type IV Collagenase

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

A-Gel VC2, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

isotype

IgG1

suitability

not suitable for Western blot

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... MMP2(4313)

General description

MMPs have a common mode of activation, a conserved amino acid sequence in the putative metal-binding active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). These MMPs and TIMPs could be expressed by either the cancer or the stromal cells. There is a cooperation between tumor and stromal cells, in particular for the production of 72-kD type IV collagenase, involved in the disruption of basement membranes. A lack of TIMP-1 expression from invasive cancer cells could also contribute to matrix destruction.

Specificity

MAB13431 recognizes proteins of 72kDa and ~66kDa which are identified as pro (latent) and active forms of matrix metalloproteinase-2 (MMP-2; also known as 72kDa collagenase IV or gelatinase A). CHEMICON MAB-13431 shows no cross-reaction with pro and active forms of other MMPs.

CELLULAR LOCALIZATION:

Cytoplasmic.

Immunogen

Epitope: pro and active form
Human APMA-activated native 72kDa Gelatinase A.

Application

Anti-MMP-2 Antibody, pro & active form, clone A-Gel VC2 is an antibody against MMP-2 for use in ELISA, IH, IH(P).
Immunohistochemistry (Frozen & fixed-paraffin embedded) 2-4 μg/mL 30 min at RT. Staining of formalin-fixed tissues is IMPROVED by boiling tissue sections in 10mM citrate buffer, pH6.0, for 10-20 min followed by cooling at RT for 20 min.

ELISA (not suitable for coating, contains BSA) 1:200-1:2000

Not suitable for Western blotting


Optimal working dilution should be determined by the end user.
Research Category
Cell Structure
Research Sub Category
MMPs & TIMPs

Linkage

Replaces: 04-1048

Physical form

10mM PBS, pH 7.4, with 0.2% BSA and 15mM sodium azide
Format: Purified

Storage and Stability

Antibody is stable for 12 months when stored at 2-8ºC

Analysis Note

Control
POSTIVE CONTROL:

Conditioned, serum-free medium from (dexametha-sone-treated) human fibrosarcoma HT-1080 or endothelial HUVEC cells. Placenta, bladder, breast and ovarian carcinomas.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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