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MABS415

Sigma-Aldrich

Anti-PPM1A, clone 7F12 Antibody

clone 7F12, from mouse

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Synonym(s):
PP2C-alpha, Protein phosphatase IA, Protein phosphatase 2C isoform alpha, PP2CA, PPM1A, PPPM1A
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

7F12, monoclonal

species reactivity

human

technique(s)

ELISA: suitable
flow cytometry: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PPM1A(5494)

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SAB1409469SAB1300999WH0005495M1
vibrant-m

MABS415

Anti-PPM1A, clone 7F12 Antibody

clone

7F12, monoclonal

clone

polyclonal

clone

polyclonal

clone

1A3-2A4, monoclonal

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

mouse

Gene Information

human ... PPM1A(5494)

Gene Information

human ... PPM1A(5494)

Gene Information

human ... PPM1E(132160)

Gene Information

human ... PPM1B(5495)

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

isotype

IgG1

isotype

-

isotype

-

isotype

IgG1κ

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General description

Protein phosphatase 1A, also known as Protein phosphatase 2C isoform alpha (PP2C-alpha) or Protein phosphatase IA, and encoded by the gene name PPM1A, is a member of the PP2C family. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis.

Immunogen

Purified recombinant fragment of human PPM1A expressed in E. Coli

Application

Anti-PPM1A, clone 7F12 Antibody is an antibody against PPM1A for use in western blotting, flow cytometry & ELISA.
Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected PPM1A in HeLa cells.

ELISA: A representative lot detected control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Research Category
Signaling
Research Sub Category
Metabolic Hormones & Receptors

Quality

Evaluated by Western Blotting in Jurkat, A431, HeLa, Raji, MCF-7 and COS7 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected PPM1A in Jurkat, A431, HeLa, Raji, MCF-7 and COS7 cell lysates.

Target description

~43 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal in PBS containing up to 0.1% sodium azide and 0.5% protein stabilizer.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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