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MABT339

Sigma-Aldrich

Anti-ZO-1 Antibody, clone 5G6.1

clone 5G6.1, from mouse

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Synonym(s):
Tight junction protein ZO-1, Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1, ZO-1
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

5G6.1, monoclonal

species reactivity

rat, human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TJP1(7082)

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This Item
SAB1306492HPA001636HPA001637
antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

5G6.1, monoclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

rat, human

species reactivity

human

species reactivity

human

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

flow cytometry: 1:10-1:50, immunohistochemistry: 1:50-1:100, western blot: 1:1000

technique(s)

immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:200-1:500, western blot: 0.04-0.4 μg/mL

technique(s)

immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:50-1:200

isotype

IgG2aκ

isotype

-

isotype

-

isotype

-

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General description

Tight junction protein ZO-1 (UniProt Q07157; also known as Tight junction protein 1, Zona occludens protein 1, Zonula occludens protein 1) is encoded by the TJP1 gene (also known as ZO1) (Gene ID 7082) in human. The tight junction (TJ) constitutes the barrier between the apical and the basolateral domains of the plasma membrane. The assembly and permeability of this barrier are dependent on the zonula occludens (ZO) membrane-associated guanylate kinase (MAGUK) proteins ZO-1, ZO-2, and ZO-3. ZO-1, a 210-225 kDa protein, is found at the submembranous domain of TJs in epithelia and endothelia. ZO-1 contains three PDZ domains (PDZ1/aa23-109; PDZ2/aa181-261; PDZ3/aa422-502) at its N-treminal end, followed by an SH3 domain (aa519-580), a GUK/GK homology domain (aa632-782), an acidic domain (aa817-894), an alpha spliced domain (aa921-1000), and a C-terminal proline-rich/PR domain. ZO-1 is a phosphoprotein and a known substrate of serine/threonine kinases ZAK and of PKC. MAPK signaling pathway regulates tyrosine phosphorylation of ZO-1, and MEK1 inhibition in Ras transformed epithelial cells is reported to result in tyrosine phosphorylation of ZO-1 and occludin. ZO-1 interacts with claudins, JAM, ZO-2, and ZO-3 through its PDZ domains, while its GK module mediates interaction with occludin. ZO-1 also binds actin cytoskeleton and actin-binding protein 4.1 through its carboxyl terminal end. In addition, ZO-1 associates with AF-6 and cingulin at the TJ, as well as with the adherens junction protein alpha-catenin and with the gap junction proteins connexins 43 and 45.

Immunogen

GST-tagged recombinant protein corresponding to human ZO-1.

Application

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected ZO-1 in human and rat kidney tissues.
Research Category
Cell Structure
Research Sub Category
Adhesion (CAMs)
This Anti-ZO-1 Antibody, clone 5G6.1 is validated for use in Western Blotting, Immunocytochemistry, Flow Cytometry, ELISA for the detection of ZO-1 .

Quality

Evaluated by Western Blotting in HCT116 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected ZO-1 in 200 µg of HCT116 cell lysate.

Target description

~195 kDa observed

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Muhammad Ali et al.
Stem cell reports, 16(9), 2320-2335 (2021-08-07)
Here, we evaluate the efficacy of cryopreserved human embryonic stem cell (hESC)-derived corneal endothelial cells (CECs) to form a functional monolayer of corneal endothelium (CE) in rabbits and monkeys. We injected cryopreserved hESC-derived CECs into the anterior chamber of rabbits
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Ischemic stroke (IS) can cause disability and death, and microglia as the immune component of the CNS can release inflammatory factors and participate in blood-brain barrier (BBB) dysfunction. This study aimed to investigate the effects of long noncoding RNA (lncRNA)
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