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Simplicon® Human iPS Cell Line

Highly characterized integration-free human induced pluripotent stem cell (iPSC) line generated using Simplicon® RNA reprogramming technology.

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Synonym(s):
Human Pluripotent Stem Cell Line, Human iPSC Line

packaging

vial of ≥1X10⁶  cells

technique(s)

cell culture | stem cell: suitable

shipped in

dry ice

storage temp.

−196°C

General description

The discovery that somatic cells could revert back to pluripotent like cells in 2006 by Shinya Yamanaka created an entirely new area of stem cell biology. Human iPS cells removed the ethical concerns associated with embryonic stem cells and allowed scientists to model human diseases that were previously impossible to model such as Alzheimer′s, Parkinson′s and Autism. Human induced pluripotent stem cells (iPSCs), have the capacity to give rise to differentiated progeny representative of all three germ layers (ectoderm, endoderm, and mesoderm). The ability to expand pluripotent cells in vitro and subject them to direct differentiation to produce specific cell types is crucial for the development of potential cell-based therapies and disease-in-a-dish cell models for research.

Simplicon® RNA Reprogramming Technology is a next generation non-integrating reprogramming system that uses a single synthetic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells. The single RNA strand contains the four reprogramming factors, OCT-4, KLF-4, SOX-2 and GLIS1 (OKSG) and enables extremely efficient reprogramming using a single transfection step without any viral intermediates or host genome integration. TheSimplicon® human iPS cell line was generated by reprogramming human foreskin fibrobalsts (SCC058) using theSimplicon® RNA Reprogramming Kit (SCR550). The cell line is integration-free, highly characterized and maintains a pluripotent phenotype over multiple passages.

Application

Highly characterized integration-free human induced pluripotent stem cell (iPSC) line generated using Simplicon® RNA reprogramming technology.

Packaging

≥1X106 cells/vial
>=1X106 cells/vial

Storage and Stability

Simplicon® human iPS cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Other Notes

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Legal Information

SIMPLICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Articles

Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.

Protocols

Step-by-step stem cell culture protocols for human induced pluripotent stem cells (iPSCs) including ips cell thawing, expanding, freezing and characterizing.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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