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XTG9-RO

Roche

X-tremeGENE 9 DNA Transfection Reagent

Polymer reagent for transfecting common cell lines

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Synonym(s):
Xtremegene, transfection, transfection reagent

grade

for molecular biology

Quality Level

form

liquid (aqueous solution)

usage

 mL (suitable for 165 transfections)

packaging

pkg of 0.4 mL (06365779001)
pkg of 1.0 mL (06365787001)
pkg of 5 × 1.0 mL (06365809001)

manufacturer/tradename

Roche

technique(s)

transfection: suitable

storage temp.

2-8°C

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form

liquid (aqueous solution)

form

liquid (aqueous solution)

form

-

form

liquid

packaging

pkg of 0.4 mL (06365779001), pkg of 1.0 mL (06365787001), pkg of 5 × 1.0 mL (06365809001)

packaging

pkg of 0.4 mL (08724105001), pkg of 1.0 mL (08724121001), pkg of 5 × 1.0 mL (08724156001)

packaging

pkg of 1 mL (04476093001 [1 mg/ml]), pkg of 5 × 1 mL (04476115001 [5 x 1 mg/ml])

packaging

-

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Roche

manufacturer/tradename

Novagen®

technique(s)

transfection: suitable

technique(s)

transfection: suitable

technique(s)

transfection: suitable

technique(s)

transfection: suitable

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

X-tremeGENE 9 DNA Transfection Reagent is a proprietary blend of lipids and other components supplied in 80% ethanol, filtered through 0.2 μm pore size membrane, and packaged in glass vials.

Application

X-tremeGENE 9 DNA Transfection Reagent is a non-liposomal multi-component reagent for experiments involving cellular analysis. Due to its extremely low cytotoxicity, minimal need for optimization, and the ability to provide high transfection efficiency in a wide range of commonly used cell lines even in the presence of serum, it is well suited for applications in all fields of cellular analysis.

X-tremeGENE 9 DNA Transfection Reagent is well suited for cellular analysis applications such as:
  • Expression of recombinant proteins for functional analysis.
  • Physiological studies of metabolic pathways.
  • Analysis of regulatory sequences using reporter gene assays.
  • Gene expression assays.
  • Cancer research studies.
  • Target evaluation.

Features and Benefits

  • Generate physiologically relevant results using a reagent with extremely low cytotoxicity for maximum post-transfection cell viability.
  • Save time and eliminate multiple handling steps; simply dilute X-tremeGENE 9 DNA Transfection Reagent, incubate with plasmid DNA, and pipet the mixture directly onto your cells (with or without serum).
  • Avoid time-consuming optimization procedures in commonly used cell lines.

Quality

Each lot of X-tremeGENE 9 DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE 9 DNA Transfection Reagent (ratio 3:1 μl/μg DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

Physical form

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE 9 DNA Transfection Reagent can be used to perform up to 6,600 transfections in 96-well plates using 3:1 ratio and up to 10,000 transfections using 2:1 ratio.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

X-tremeGENE is a trademark of Roche

Pictograms

FlameExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

334.4 °F

Flash Point(C)

168 °C


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Protocols

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

Co-transfection of Plasmid DNA

Transient co-transfection of plasmids is a method that is commonly employed for cellular protein-protein interaction studies, transcription factor studies, and gene knockdown studies using shRNA encoding plasmids.

X-tremeGENE™ HP DNA Transfection Reagent Protocol

Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents

Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents

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Transfection Reagent Selection Guide

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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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