MilliporeSigma
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14366C

SAFC

EX-CELL® Advanced CHO Fed-batch Medium

liquid

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NACRES:
NA.75

sterility

sterile-filtered

Quality Level

form

liquid

technique(s)

cell culture | mammalian: suitable (suspension)

components

L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

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This Item
24366CC636614340C
SAFC

SAFC

C6366

EX-CELL® CHO Cloning Medium

form

liquid

form

dry powder

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | mammalian: suitable

components

L-glutamine: no

components

-

components

L-glutamine: no
NaHCO3: yes

components

glucose: 3.45 g/L
L-glutamine: no
NaHCO3: 1.6 g/L
HEPES: 7.5 mM
phenol red: yes

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

EX-CELL® AdvancedCHO Fed-batch Medium is chemically-defined and contains no animal derived components. This newest platform from SAFC has been specifically developed to support growth and productivity across a diverse set of Chinese Hamster Ovary (CHO) cells in fed-batch cultures while still allowing for flexibility in the adjustment of protein quality attributes. This contemporary fed-batch platform was developed using a strategy of Multivariate Analysis to establish correlations in which the formulation components influence critical process and product attributes such as titer, fold expansion, receptor expression, and glycosylation. This platform contains no hypoxanthine and thymidine, L-glutamine, phenol red or 2-mercaptoethanol (2-ME). This medium is intended to be a part of a fed-batch platform, and used with EX-CELL® Advanced CHO Feed 1 (24367C - with glucose or 24368C - without glucose).

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany

related product

Product No.
Description
Pricing

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Weiyi Liu et al.
Biotechnology progress, 37(1), e3061-e3061 (2020-08-05)
Antibody-dependent cellular cytotoxicity (ADCC) is the primary mechanism of actions for several marketed therapeutic antibodies (mAbs) and for many more in clinical trials. The ADCC efficacy is highly dependent on the ability of therapeutic mAbs to recruit effector cells such

Articles

This case study walks through optimizing feed and media for upstream bioprocessing and how it translates to large-scale bioreactors.

In this study, nine commercially available, chemically defined media and feed combinations from different vendors were assessed for producing monoclonal antibody in Chinese hamster ovary (CHO) cells.

This application note describes the benefits and performance of Cellvento® 4CHO-X Expansion Medium designed for multiple cell expansion steps from vial thaw to N-1 stages to generate higher biomass for inoculating fed-batch production bioreactors

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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