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EX-CELL® CD HEK293 Viral Vector Medium

Chemically defined, animal component-free, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
HEK293/HEK293T medium for viral vector production

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable (suspension)

components

L-glutamine: no

shipped in

wet ice

storage temp.

2-8°C

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EX-CELL® CD Insect Cell Medium Chemically defined, powder, suitable (for insect cell culture)

24381C

EX-CELL® CD Insect Cell Medium

form

liquid

form

liquid

form

dry powder

form

-

technique(s)

cell culture | mammalian: suitable (suspension)

technique(s)

cell culture | insect: suitable

technique(s)

cell culture | insect: suitable

technique(s)

-

components

L-glutamine: no

components

NaHCO3: no
L-glutamine: yes

components

L-glutamine: yes

components

-

shipped in

wet ice

shipped in

ambient

shipped in

ambient

shipped in

liquid nitrogen

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−196°C

General description

EX-CELL CD HEK293 Viral Vector Medium is an animal origin-free, chemically defined cell culture medium for clinical and commercial manufacturing of viral vectors for gene and cell therapies. The medium is intended for processes implementing (polyethylenimine) PEI transfection reagents. EX-CELL CD HEK293 Viral Vector Medium is part of the VirusExpress® Lentiviral Production Platform and the medium of choice for VirusExpress 293T Lentiviral Production Cells (VP001). The medium is also suitable as a standalone solution for other HEK293 and HEK293T cell lines for AAV and lentiviral manufacturing. EX-CELL CD HEK293 Viral Vector Medium has been formulated to support high suspension cell densities with minimal clumping, high transient transfection efficiency when used with PEI, and high viral titers in shake flask and stirred tank bioreactor formats. Viral vector production using this medium is a scalable, suspension process that does not require a media exchange or feed post-transfection.

EX-CELL CD HEK293 Viral Vector Medium has been designed with long-term needs for manufacturing in mind, with a sustainable raw material supply chain, custom packaging options, and careful material and manufacturing process selection. The product is produced in an ISO9001-certified facility following the principles of cGMP according to IPEC-PQG.

Application

EX-CELL CD HEK293 Viral Vector Medium was developed to support VirusExpress Production Platforms. It performs well within the platform as well as with other HEK293 clones. The following white paper demonstrates the use of one of the prototype media formulations in developing a scalable, transient transfection-based suspension process for lentiviral vector production using the VirusExpress 293T Lentiviral Production cells:

Legal Information

EX-CELL is a registered trademark of Merck KGaA, Darmstadt, Germany
VirusExpress is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Articles

Scaling up viral vector production using adherent cell culture systems is challenging. Learn how suspension cell culture systems benefit large-scale bioprocessing.

A step-by-step overview of suspension-based, transient transfection bioreactor process development and scaleup of lentivirus production.

To address scalability challenges of AAV manufacturing, we developed an HEK293 suspension cell line that can be used across many serotypes. Get the data in this article.

Optimizing the upstream portion of gene therapy production sets the stage for successful manufacturing by maximizing viral vector titers. To increase upstream titers and process productivity, manufacturers must partner with upstream technology experts who work with HEK293, HEK293T and Sf9 cells. Draw on our experience today to speed your gene therapy to patients.

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