MilliporeSigma
All Photos(1)

Documents

21888

Sigma-Aldrich

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

Synonym(s):
CFSE
Empirical Formula (Hill Notation):
C29H19NO11
CAS Number:
Molecular Weight:
557.46
MDL number:
PubChem Substance ID:
NACRES:
NA.32

product line

BioReagent

Quality Level

Assay

≥90% (HPLC)

form

solid

mp

152-154 °C (lit.)

solubility

DMF: soluble
DMSO: soluble

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)c1.CC(=O)Oc7ccc8c(Oc9cc(OC(C)=O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)ON%12C(=O)CCC%12=O)c7

InChI

1S/2C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34;1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h2*3-8,11-13H,9-10H2,1-2H3

InChI key

LZFDYQHOQDFMOA-UHFFFAOYSA-N

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
889975304875068
assay

≥90% (HPLC)

assay

-

assay

-

assay

≥90% (HPCE)

form

solid

form

powder

form

-

form

powder

mp

152-154 °C (lit.)

mp

-

mp

-

mp

-

solubility

DMF: soluble, DMSO: soluble

solubility

DMF: 2 mg/mL, clear, acetonitrile: soluble

solubility

DMF: soluble, acetonitrile: soluble

solubility

DMSO: soluble

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)

fluorescence

λex 543 nm; λem 575 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 543 nm; λem 578 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 380 nm; λem 407 nm in 0.1 M phosphate pH 7.0

General description

5-(and-6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is a fluorescent dye, which is membrane permeable. It labels cells by covalently binding to intracellular molecules. CFSE is used to track lymphocyte migration and proliferation.

Application

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester (CFSE) is an amine reactive fluorescent probe used to stain cells, especially immune cells, for analysis in applications such as flow cytometry. Forms green fluorescent conjugates on deacetylation.
5-Carboxyfluorescein diacetate N-succinimidyl ester has been used:
  • to label cells for flow cytometry
  • in lymphocyte proliferation assay
  • to determine the intracellular or intra-organellar pH in chloroplasts
  • to label Leishmania amazonensis and Leishmania major

Amine-reactive label for long term tracing of cells. Forms green fluorescent conjugates on deacetylation.

Biochem/physiol Actions

5(6)-Carboxyfluorescein diacetate N-succinimidyl ester is used as an indicator for esterase activity.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

related product

Product No.
Description
Pricing

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

M Bronner-Fraser
The Journal of cell biology, 101(2), 610-617 (1985-08-01)
The possible role of a 140-kD cell surface complex in neural crest adhesion and migration was examined using a monoclonal antibody JG22, first described by Greve and Gottlieb (1982, J. Cell. Biochem. 18:221-229). The addition of JG22 to neural crest
A Reliable and Non-destructive Method for Monitoring the Stromal pH in Isolated Chloroplasts Using a Fluorescent pH Probe
Su PH and Lai YH
Frontiers in Plant Science, 8, 2079-2079 (2017)
WNT5A transforms intestinal CD8αα+ IELs into an unconventional phenotype with pro-inflammatory features
Zhao D, et al.
BMC Gastroenterology, 15(1), 173-173 (2015)
Microbial Food Safety Along the Dairy Chain (2017)
Memory CD4+ T cells are suppressed by CD8+ regulatory T cells in vitro and in vivo
Long X, et al.
American Journal of Translational Research, 9(1), 63-63 (2017)

Articles

Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service