Skip to Content
MilliporeSigma
All Photos(1)

Documents

46043

Sigma-Aldrich

Ethidium homodimer

suitable for fluorescence, ~90% (HPCE)

Synonym(s):

EtDi, EthD-1

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C46H50Cl4N8
CAS Number:
61926-22-5
Molecular Weight:
856.75
EC Number:
263-325-9
MDL number:
MFCD00210948
UNSPSC Code:
12161505
PubChem Substance ID:
329757072
NACRES:
NA.32

assay

~90% (HPCE)

form

solid

mp

≥250 °C (lit.)

solubility

DMF: soluble
DMSO: soluble
H2O: soluble

fluorescence

λex 493 nm in H2O
λex 528 nm; λem 617 nm in TE buffer; DNA

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

Cl.Cl.[Cl-].[Cl-].Nc1ccc2c(c1)c(-c3ccccc3)[n+](CCCNCCNCCC[n+]4c(-c5ccccc5)c6cc(N)ccc6c7ccc(N)cc47)c8cc(N)ccc28

InChI

1S/C46H46N8.4ClH/c47-33-13-17-37-39-19-15-35(49)29-43(39)53(45(41(37)27-33)31-9-3-1-4-10-31)25-7-21-51-23-24-52-22-8-26-54-44-30-36(50)16-20-40(44)38-18-14-34(48)28-42(38)46(54)32-11-5-2-6-12-32;;;;/h1-6,9-20,27-30,49-52H,7-8,21-26,47-48H2;4*1H

InChI key

ZKAJUKABUUJRLB-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Application

Ethidium homodimer (EtDi, EthD-1) is used as a staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. EthD-1 does not cross intact/viable cell membranes and can be used to test cell viability.
Staining dye for ssDNA, dsDNA, RNA, oligonucleotides, and triplex DNA. It will not cross intact cell membranes and can be used to test cell viability.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Reagent for the fluorimetric detection of nucleic acids

signalword

Danger

Hazard Classifications

Carc. 2 - Eye Dam. 1 - Skin Corr. 1B - Water-react 3

Storage Class

4.3 - Hazardous materials which set free flammable gases upon contact with water

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Susanne Radonjic-Hoesli et al.
The Journal of allergy and clinical immunology, 140(6), 1632-1642 (2017-04-17)
Eosinophils are a subset of granulocytes that can be involved in the pathogenesis of different diseases, including allergy. Their effector functions are closely linked to their cytotoxic granule proteins. Release takes place through several different mechanisms, one of which is
Ethidium dimer: a new reagent for the fluorimetric determination of nucleic acids.
J Markovits et al.
Analytical biochemistry, 94(2), 259-264 (1979-04-15)
Nai-Chen Cheng et al.
Acta biomaterialia, 51, 258-267 (2017-01-31)
Adipose-derived stem cells (ASCs) secrete several angiogenic growth factors and can be applied to treat ischemic tissue. However, transplantation of dissociated ASCs has frequently resulted in rapid cell death. Therefore, we aimed to develop a thermosensitive chitosan/gelatin hydrogel that is
Takeichiro Nakane et al.
Scientific reports, 7, 45641-45641 (2017-04-04)
The current study describes a scalable, porous large-format engineered cardiac tissue (LF-ECT) composed of human induced pluripotent stem cells (hiPSCs) derived multiple lineage cardiac cells with varied 3D geometries and cell densities developed towards the goal of scale-up for large
X R Mao et al.
Cell death & disease, 7, e2113-e2113 (2016-02-26)
Gain-of-function mutations in the mouse nicotinamide mononucleotide adenylyltransferase type 1 (Nmnat1) produce two remarkable phenotypes: protection against traumatic axonal degeneration and reduced hypoxic brain injury. Despite intensive efforts, the mechanism of Nmnat1 cytoprotection remains elusive. To develop a new model
View All Related Papers

Related Content

DNA Detection

Nancy-520 for DNA Detection and Quantitation

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service