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646563

Supelco

DL-Dithiothreitol solution

1 M in H2O

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Synonym(s):
(±)-threo-1,4-Dimercapto-2,3-butanediol solution, DTT
Empirical Formula (Hill Notation):
C4H10O2S2
CAS Number:
Molecular Weight:
154.25
Beilstein/REAXYS Number:
1719757
MDL number:
PubChem Substance ID:
NACRES:
NA.25

form

liquid

Quality Level

reaction suitability

reagent type: reductant

concentration

1 M in H2O

density

1.04 g/mL at 20 °C
1.035 g/mL at 25 °C

SMILES string

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

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This Item
D97794381643815
vibrant-m

646563

DL-Dithiothreitol solution

-
vibrant-m

D9779

DL-Dithiothreitol

-
vibrant-m

43816

DL-Dithiothreitol solution

Premium Grade
vibrant-m

43815

DL-Dithiothreitol

Premium Grade
reaction suitability

reagent type: reductant

reaction suitability

reagent type: reductant

reaction suitability

reagent type: reductant

reaction suitability

reagent type: reductant

density

1.04 g/mL at 20 °C, 1.035 g/mL at 25 °C

density

-

density

1.04 g/mL at 20 °C

density

-

form

liquid

form

powder

form

liquid

form

powder

concentration

1 M in H2O

concentration

-

concentration

~1 M in H2O

concentration

-

General description

Dithiothreitol (DTT) is used in proteomics applications to maintain sulfhydryl (-SH) groups in the reduced state and for quantitative reduction of disulfide (-S-S-) groups. It is a frequently used reagent in buffers due to its ability to reduce the oxidation state of a protein sample and preserves enzymatic activity. DTT is oxidized to the cyclic disulfide during the reduction of other disulfides in the solution.
Dithiothreitol is an effective reducing agent for protein analysis. The sealed-ampule format is convenient and easy to use when preparing samples for electrophoresis or other applications and avoids waste and loss of potency.

Application

DL-Dithiothreitol solution has been used:
  • in western blot analysis
  • as a supplement in lysis buffer to study its effects on sperm lysis
  • as a component of cold homogenization buffer, and chilled wash buffer for the isolation of nuclei from frozen rat tissues
  • in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), chromatography, modification of cysteine-containing compounds

Features and Benefits

Low toxicity and reduced odor compared to other thiol compounds.

accessory

Product No.
Description
Pricing

pictograms

Corrosion

signalword

Danger

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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