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806552

SAFC

Phosphate Buffered Saline

pH 7.4, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Phosphate buffered saline, PBS

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About This Item

MDL number:
MFCD00131855
UNSPSC Code:
12352207
NACRES:
NA.75

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

pH

7.4

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General description

Phosphate-buffered saline (PBS) is a buffer solution used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate. The osmolality and ion concentrations of the solutions match those of the human body (isotonic) and are non-toxic to most cells.

Application

Phosphate-buffered saline has been used:
  • as a reagent in the isolation of human endometrial stem cell/stromal cells (hEnSCs) from the endometrium or menstrual blood
  • has also been used to wash cells during immunofluorescence staining
  • as a solvent for the glucose administered orally in the Glucose Tolerance Tests (GTTs)

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

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Shangang Zhao et al.
Molecular metabolism, 37, 100995-100995 (2020-04-15)
Hyperleptinemia per se is sufficient to promote leptin resistance in the obese state. Leptin sensitivity can be restored by reducing circulating leptin levels within a physiologically healthy range and is a viable antiobesity and antidiabetic strategy. However, a previous study
Human Endometrial Stem Cell Isolation from Endometrium and Menstrual Blood
Tavakol S, et al.
Molecular Neurobiology (2016)
L R Gabbert et al.
Journal of applied microbiology, 129(5), 1092-1101 (2020-05-08)
Develop an effective laboratory method to consistently recover viral loads from porous concrete coupons sufficient for disinfectant efficacy testing. Investigate the role of concrete matrix pH on the recovery of foot-and-mouth disease virus (FMDV) and African Swine Fever virus (ASFV)
Pinkal D Patel et al.
Molecular vision, 26, 434-444 (2020-06-23)
Chronic elevation of intraocular pressure (IOP) is a major risk factor associated with primary open angle glaucoma (POAG), a common form of progressive optic neuropathy that can lead to debilitating loss of vision. Recent studies have identified the role of
Dieter Fink et al.
PloS one, 14(11), e0224576-e0224576 (2019-11-08)
Intra-bone marrow transplantation (IBMT) has been adapted for mouse models to improve the seeding efficiency of transplanted hematopoietic stem and progenitor cells. Commonly used injection volumes for IBMT into the tibia differ between 10 and 40 μL even though considerable
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