Skip to Content
MilliporeSigma
All Photos(1)

Documents

83264

Sigma-Aldrich

HEPES solution

1 M in H₂O, liquid

Synonym(s):

HEPES solution, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

Sign Into View Organizational & Contract Pricing


About This Item

CAS Number:
MDL number:
UNSPSC Code:
12161700
eCl@ss:
32129211
PubChem Substance ID:
NACRES:
NA.25

product name

HEPES buffer solution, 1 M in H2O

form

liquid

Quality Level

concentration

1 M in H2O
238 g/L HEPES in H2O

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

5.5±0.5

useful pH range

6.8-8.2

density

1.07 g/mL at 20 °C

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:
  • As a component of SAPI medium used for the culturing of S. Typhymurium inoculum
  • To prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMI
  • As a component of dissection buffer for culturing rat cortical neurons
  • Along with MgCl2, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses
  • As a component of Danieu′s aquarium water used in agarose gel preparation

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Novel membrane-based electrochemical sensor for real-time bio-applications.
Alatraktchi FA, et al.
Sensors, 14(11), 22128-22139 (2014)
Cultivation of Salmonella enterica serovar Typhimurium in a norepinephrine-containing medium alters in vivo tissue prevalence in swine.
Toscano MJ, et al.
Journal of experimental animal science, 43 , 329-338 (2007)
Phagocytosis Assay of Microglia for Dead Neurons in Primary Rat Brain Cell Cultures.
Zhao X, et al.
Bio-protocol, 6(8) (2016)
Group 2 innate lymphoid cells express functional NKp30 receptor inducing type 2 cytokine production.
Salimi M, et al.
Journal of Immunology, 196(1), 45-54 (2016)
"Hypoxia-induced metastasis model in embryonic zebrafish.
Rouhi P, et al.
Nature Protocols, 5(12), 1911-1911 (2010)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service