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83264

Sigma-Aldrich

HEPES buffer solution

1 M in H2O

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Synonym(s):
HEPES solution, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
CAS Number:
MDL number:
eCl@ss:
32129211
PubChem Substance ID:
NACRES:
NA.25

form

liquid

Quality Level

concentration

1 M in H2O
238 g/L HEPES in H2O

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

5.5±0.5

useful pH range

6.8-8.2

density

1.07 g/mL at 20 °C

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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This Item
H3537H23830H7523
HEPES buffer solution 1 M in H2O

Sigma-Aldrich

83264

HEPES buffer solution

-
HEPES solution BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

Sigma-Aldrich

H3537

HEPES solution

Premium Grade
HEPES reagent grade, ≥99% (titration), crystalline

Sigma-Aldrich

H23830

HEPES

Essential Grade
HEPES BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

Sigma-Aldrich

H7523

HEPES

Premium Grade
concentration

1 M in H2O, 238 g/L HEPES in H2O

concentration

1 M

concentration

-

concentration

-

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

impurities

Bioburden, tested, DNase, RNase, Protease, Nickase, free, endotoxin, tested

impurities

-

impurities

Insoluble matter, passes filter test

pH

5.5±0.5

pH

5.0-6.0

pH

-

pH

5.0-6.5 (1 M in H2O)

density

1.07 g/mL at 20 °C

density

-

density

-

density

-

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

diagnostic assay manufacturing

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:
  • As a component of SAPI medium used for the culturing of S. Typhymurium inoculum
  • To prepare differentiation medium for PC12 cells. It is also a component of culture medium RPMI
  • As a component of dissection buffer for culturing rat cortical neurons
  • Along with MgCl2, KCl and Spermine in hypotonic buffer in mGRASP method to detect mammalian synapses
  • As a component of Danieu′s aquarium water used in agarose gel preparation

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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T1503
Product Number
-
25G
Pack Size/Quantity

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

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Novel membrane-based electrochemical sensor for real-time bio-applications.
Alatraktchi FA, et al.
Sensors, 14(11), 22128-22139 (2014)
Cultivation of Salmonella enterica serovar Typhimurium in a norepinephrine-containing medium alters in vivo tissue prevalence in swine.
Toscano MJ, et al.
Journal of experimental animal science, 43 , 329-338 (2007)
Phagocytosis Assay of Microglia for Dead Neurons in Primary Rat Brain Cell Cultures.
Zhao X, et al.
Bio-protocol, 6(8) (2016)
Group 2 innate lymphoid cells express functional NKp30 receptor inducing type 2 cytokine production.
Salimi M, et al.
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"Hypoxia-induced metastasis model in embryonic zebrafish.
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