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93017

Sigma-Aldrich

SSC Buffer 20x

for Northern and Southern blotting, solution

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Synonym(s):
20x SSC
NACRES:
NA.25

grade

for molecular biology

Quality Level

product line

BioReagent

form

solution

technique(s)

Southern blotting: suitable

impurities

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

pH

7.0±0.2

density

1.16 g/mL at 20 °C

storage temp.

room temp

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S0902B6185B6429
Sigma-Aldrich

Sigma-Aldrich

93017

SSC Buffer 20x

Sigma-Aldrich

Sigma-Aldrich

S0902

SSC Buffer

product line

BioReagent

product line

-

product line

-

product line

-

form

solution

form

powder blend

form

solution

form

solution

technique(s)

Southern blotting: suitable

technique(s)

-

technique(s)

-

technique(s)

Southern blotting: suitable

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

impurities

-

impurities

DNase, RNase and nickase, none detected

impurities

-

pH

7.0±0.2

pH

6.8-7.2 (20-25 °C, 263 mg/L in water)

pH

8.0-8.5 (20-25 °C)

pH

-

General description

SSC Buffer 20x Concentrate is a standard reagent in Southern and Northern blotting procedures. This concentrated formula can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

Application

Suitable for:
  • Preparing nucleic acids for hybridization
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for Northern and Southern blots

Features and Benefits

  • Convenient ready-to-use liquid
  • Multi-purpose use in hybridizations
  • Easily diluted to any concentration needed

Components

SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Protocols

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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