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93290

Sigma-Aldrich

TRIS borate – EDTA buffer solution

for Northern and Southern blotting, solution

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Synonym(s):
TBE buffer
eCl@ss:
32129211
NACRES:
NA.25

grade

for molecular biology

Quality Level

description

long run, concentrate

product line

BioReagent

form

solution

quality

10× concentrate
ready-to-use solution

impurities

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

pH

8.6-9.0

storage temp.

room temp

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This Item
T4415T928593283
Sigma-Aldrich

93290

TRIS borate – EDTA buffer solution

Essential+ Grade
Tris-Borate-EDTA buffer BioReagent, suitable for electrophoresis, 10× concentrate

T4415

Tris-Borate-EDTA buffer

Essential+ Grade
Tris-EDTA buffer solution 100X concentrate for Northern and Southern blotting

T9285

Tris-EDTA buffer solution

-
Tris-EDTA buffer solution BioUltra, for molecular biology, pH 8.0

93283

Tris-EDTA buffer solution

Premium Grade
Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

product line

BioReagent

product line

BioReagent

product line

-

product line

BioUltra

form

solution

form

solution

form

liquid

form

liquid

pH

8.6-9.0

pH

-

pH

7.8-8.2 (1 × in water)

pH

8.0±0.2 (25 °C)

storage temp.

room temp

storage temp.

-

storage temp.

room temp

storage temp.

-

General description

TBE (Tris/Borate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. The TBE solution is effective under slightly basic conditions, which keeps DNA deprotonated , water-soluble, and protected from degradation. This concentrate can be easily diluted to 1x or 0.5x before use (with molecular biology grade water).

Application

Suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures.

Components

The TBE 10x liquid contains 1.3M TRIS, 450 mM boric acid and 25 mM EDTA in water.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Repr. 1B

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter


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Boric acid suitable for electrophoresis, ≥99.5%

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L. Johnston-Dow et al.
Biotechniques, 5, 754-754 (1987)

Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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