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A2228

Sigma-Aldrich

Monoclonal Anti-β-Actin antibody produced in mouse

clone AC-74, purified immunoglobulin, buffered aqueous solution

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Synonym(s):
Actin Antibody Sigma, Beta Actin Antibody - Monoclonal Anti-β-Actin antibody produced in mouse, Beta Actin Antibody Sigma, Beta Actin Sigma Antibody, Sigma Actin Antibody, Sigma Beta Actin Antibody
MDL number:
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AC-74, monoclonal

form

buffered aqueous solution

mol wt

antigen 42 kDa

species reactivity

rabbit, sheep, cat, guinea pig, bovine, mouse, chicken, wide range, Hirudo medicinalis, canine, Drosophila, carp, pig, rat, human

should not react with

Dictyostelium discoideum

concentration

~2 mg/mL

technique(s)

immunocytochemistry: 10-40 μg/mL using human or chicken fibroblasts
immunohistochemistry: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using total cell extracts of human or chicken fibroblasts

isotype

IgG2a

UniProt accession no.

application(s)

research pathology

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)

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This Item
A5316A5441A1978
Gene Information

human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)

Gene Information

human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)

Gene Information

human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)

Gene Information

human ... ACTB(60)
mouse ... Actb(11461)
rat ... Actb(81822)

clone

AC-74, monoclonal

clone

AC-74, monoclonal

clone

AC-15, monoclonal

clone

AC-15, monoclonal

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

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General description

ACTB (actin β) gene codes for β-actin. It is a cytoskeletal housekeeping protein. It is present in the cytoplasm. This gene is located on human chromosome 7p22.1.
Recognizes an epitope located on the N-terminal end of the β-isoform of actin. It specifically labels β-actin in a wide variety of tissues and species. In immunofluorescent staining of chicken gizzard ultrathin cryosections, the antibody labels the dense bodies, the longitudinal channels linking consecutive dense bodies that are also occupied by desmin and the membrane-associated dense plaque. The antibody does not react with adult cardiac or skeletal muscle (besides traces due to contamination of the sample with non-muscle cells) or with β-actin expressing cells in Dictyostelium discoideum amoeba. The epitope recognized by the antibody is resistant to formalin-fixation and paraffin-embedding. B5, methacarn, ethanol or Bouin′s solutions may be also used as fixatives. The antibody can be used as probes for β-actin as an internal control in immunoblotting.

Immunogen

slightly modified β-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Application

Monoclonal Anti-β-Actin antibody has been used in western blot and two-dimensional gel immunoblot.
Monoclonal anti-β-actin antibody can be used for immunocytochemistry (10-40μg/mL using human or chicken fibroblasts), immunohistochemistry and microarray analysis.
Monoclonal mouse anti-actin antibody was used as a loading control for western blot analysis of immunoprecipitated proteins from rat dorsal root ganglion cocultures.
Western blot analysis of MDCK cell lysates were performed using monoclonal anti-actin antibody as a primary antibody.

Biochem/physiol Actions

Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin.
β-Actin modulates cell growth, migration and the G-actin pool. Mutations in ACTB leads to pleiotropic developmental disorder.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at -20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Other Notes

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Immune Pathology Associated with Altered Actin Cytoskeleton Regulation.
Dilki W, et al.
Autoimmunity, 43(1), 64-75 (2010)
Isoflurane induces hippocampal cell injury and cognitive impairments in adult rats.
Daowei L and Zhiyi Z
Neuropharmacology, 37(8), 1354-1359 (2011)
Yuyu Yang et al.
Biochimica et biophysica acta. Gene regulatory mechanisms, 1862(8), 834-845 (2019-06-04)
Prostate cancer malignancies are intimately correlated with deregulated fatty acid metabolism. The underlying epigenetic mechanism is not fully understood. In the present study we investigated the mechanism whereby the chromatin remodeling protein BRG1 regulates the transcription of long-chain fatty acid
Over-expression of the BRMS1 family member SUDS3 does not suppress metastasis of human cancer cells
Alexandra CS, et al.
Cancer Letters, 276(1), 32-37 (2009)
β-Actin specifically controls cell growth, migration, and the G-actin pool
Tina M B, et al.
Molecular Biology of the Cell, 22(21) (2011)

Articles

The field of proteomics is continually looking for new ways to investigate protein dynamics within complex biological samples. Recently, many researchers have begun to use RNA interference (RNAi) as a method of manipulating protein levels within their samples, but the ability to accurately determine these protein amounts remains a challenge. Fortunately, over the past decade, the field of proteomics has witnessed significant advances in the area of mass spectrometry. These advances, both in instrumentation and methodology, are providing researchers with sensitive assays for both identification and quantification of proteins within complex samples. This discussion will highlight some of these methodologies, namely the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.

High titer lentiviral particles including beta-actin, alpha-tubulin and vimentin used for live cell analysis of cytoskeleton structure proteins.

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