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A3403

Sigma-Aldrich

α-Amylase from Bacillus licheniformis

greener alternative

Type XII-A, saline solution, ≥500 units/mg protein (biuret)

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Synonym(s):
Termamyl® 120, 1,4-α-D-Glucan-glucanohydrolase
CAS Number:
Enzyme Commission number:
MDL number:
eCl@ss:
32160410
NACRES:
NA.54

type

Type XII-A

Quality Level

form

saline solution

specific activity

≥500 units/mg protein (biuret)

mol wt

57.6 kDa

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

greener alternative category

storage temp.

2-8°C

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1 of 4

This Item
A3306A4551A4862
vibrant-m

A3306

α-Amylase, heat-stable

vibrant-m

A4862

α-Amylase from Bacillus sp.

mol wt

57.6 kDa

mol wt

-

mol wt

58-62 kDa

mol wt

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

form

saline solution

form

solution

form

lyophilized powder

form

liquid

Quality Level

200

Quality Level

300

Quality Level

200

Quality Level

200

type

Type XII-A

type

-

type

-

type

-

General description

α-Amylase is a member of the endo-amylase family. Its molecular weight is 57.6 kDa. α-Amylase is composed of three functional domains namely A, B and C. Domain A is a barrel-shaped (β/α)8 structure and is the largest domain. Domain B is linked to domain A via disulfide bond and is present between domain A and C. Domain C is composed of β-sheets and is connected to domain A via a polypeptide chain.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Application

α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. Product A3403 is from Bacillus licheniformis and is type XII-A. α-Amylase, from Sigma, has been used in various plant studies, such as metabolism studies in Arabidopsis .

Biochem/physiol Actions

α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose.

Caution

Reported to be heat stable at temperatures as high as ∼90 °C.

Unit Definition

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

Physical form

Aqueous solution containing sodium chloride and sucrose

Legal Information

A product of Novozyme Corp.
Termamyl is a registered trademark of Novozymes Corp.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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Protocols

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

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