Skip to Content
MilliporeSigma
All Photos(1)

Documents

A4338

Sigma-Aldrich

Anti-Bovine Albumin (BSA)−Agarose antibody produced in rabbit

IgG fraction of antiserum, PBS suspension

Synonym(s):

Anti-Albumin, bovine-Agarose, Anti-BSA Agarose

Sign Into View Organizational & Contract Pricing


About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

conjugate

agarose conjugate

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

PBS suspension

species reactivity

bovine

extent of labeling

2.0-2.4 mg antibody per mL settled resin

technique(s)

immunoprecipitation (IP): suitable

capacity

1.5-3.0 mg/mL, resin binding capacity (bovine serum albumin)

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

mouse ... Alb(280717)

Related Categories

General description

Albumin, the major protein produced by liver cells, represents more than half of the total protein content of human serum. Many other body fluids also contain albumin. Three major functions for serum albumin have been proposed: maintenance of osmotic pressure, transportation of a variety of substances and an endogenous source of amino acids. The primary sites of albumin degradation are not known, but the protein can be metabolized by almost every organ in the body. Determination of serum albumin levels is a widely used screening test in clinical medicine. A decrease in serum albumin levels may indicate disease states such as malnutrition, cirrhosis, nephrotic syndrome, diabetes, gastrointestinal and hepatic diseases, thermal burns and pulmonary disease.
Rabbit polyclonal anti-Bovine Serum Albumin (BSA)–Agarose reacts with bovine albumin.
Serum albumins are soluble constituents of circulatory system. Bovine serum albumin (BSA) is homologous to human serum albumin (HSA). BSA consists of three homologous domains (I, II, III), that are separated into nine loops by disulfide bonds.

Immunogen

purified BSA from Cohn Fraction V

Application

Heparin-binding Merozoite antigens were detected by heparin-BSA binding assays using rabbit anti-BSAas the primary after plates were incubated with heparin-BSA on merozoite coated plates.
May be used for immunoprecipitation and immunoaffinity purification of Bovine Serum Albumin (BSA) from solutions.
Rabbit Anti-Bovine Albumin (BSA)-Agarose antibody has been used as a negative control in several immunoassays.
Used for immunoprecipitation and immunoaffinity purification of BSA from solutions.
Anti-Bovine Albumin (BSA)-Agarose antibody produced in rabbit has been used in mass spectrometry.

Biochem/physiol Actions

Serum albumin acts as a depot protein.

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Preparation Note

Prepared by conjugating the IgG fraction of anti-bovine serum albumin (BSA) to cyanogen bromide-activated agarose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Product Selector Tool.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Raj Mutharasan
Methods in molecular biology (Clifton, N.J.), 504, 73-82 (2009-01-23)
In this chapter, method of fabricating a cantilever biosensors and their use in measuring the presence of a protein is described. There are many variations in construction of a cantilever sensor. A simple and an easy version is described in
Interaction of flavonoids with bovine serum albumin: a fluorescence quenching study
Papadopoulou A, et al.
Journal of Agricultural and Food Chemistry, 53(1), 158-163 (2005)
Tumor promoter PMA enhances kindlin-2 and decreases vimentin recruitment into cell adhesion sites
Salmela M, et al.
The International Journal of Biochemistry & Cell Biology, 78, 22-30 (2016)
Lonnie J Lucas et al.
Colloids and surfaces. B, Biointerfaces, 49(2), 106-111 (2006-04-20)
Manufacturers of latex immunoassays have typically added surfactants to improve detection sensitivity and prevent non-specific aggregation of microspheres, which may cause both false positives and negatives during diagnostic testing. There is also growing interest in conducting immunoassays in smaller volumes
Lisa M Bank et al.
Development (Cambridge, England), 139(24), 4666-4674 (2012-11-23)
This study is the first to demonstrate that macrophage migration inhibitory factor (MIF), an immune system 'inflammatory' cytokine that is released by the developing otocyst, plays a role in regulating early innervation of the mouse and chick inner ear. We

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service