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A5043

Sigma-Aldrich

Adenosine Deaminase from bovine spleen

Type X, buffered aqueous glycerol solution, ≥130 units/mg protein

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Synonym(s):
ADA, adenosine aminohydrolase, Adenosine aminohydrolase
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54

biological source

bovine spleen

Quality Level

type

Type X

form

buffered aqueous glycerol solution

specific activity

≥130 units/mg protein

mol wt

32.5-33 kDa

cation traces

NH4+: ≤10 μg/mg protein

UniProt accession no.

foreign activity

alkaline phosphatase ≤0.01%
guanase ≤0.01%
nucleoside phosphorylase ≤0.1%

storage temp.

2-8°C

Gene Information

cow ... ADA(280712)

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This Item
A5168P9041L1006
specific activity

≥130 units/mg protein

specific activity

150-200 units/mg protein

specific activity

≥5.0 units/mg protein

specific activity

≥400 units/mg protein

biological source

bovine spleen

biological source

bovine spleen

biological source

-

biological source

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

foreign activity

alkaline phosphatase ≤0.01%, nucleoside phosphorylase ≤0.1%, guanase ≤0.01%

foreign activity

5′-AMP deaminase ≤0.01%, alkaline phosphatase ≤0.01%, guanase ≤0.01%, nucleoside phosphorylase ≤0.1%

foreign activity

5′-Nucleotidase ≤1% of base activity

foreign activity

glutamic-pyruvic transaminase and glutamic-oxalacetic transaminase ≤0.02%, pyruvate kinase, myokinase and α-glycerophosphate dehydrogenase ≤0.01%

form

buffered aqueous glycerol solution

form

ammonium sulfate suspension

form

lyophilized powder

form

buffered aqueous glycerol solution

General description

Molecular weight: 32.5-33 kDa

pI: 4.85

Application

Adenosine Deaminase from bovine spleen has been used in the immobilization on biostrip for stability and catalytic studies.
Adenosine deaminase is useful in various molecular biology assays, such as glycerol release assays . Adenosine deaminase is a potential target for treatments of combined immunodeficiency disease.

Biochem/physiol Actions

Adenosine deaminase is a purine catabolic enzyme which catalyzes the deamination of adenosine and 2′-deoxyadenosine with approximately equal specificity. It is ubiquitous in mammalian tissue, and deficiency in adenosine deaminase has been associated with severe combined immunodeficiency disease.

Unit Definition

One unit will deaminate 1.0 μmole of adenosine to inosine per min at pH 7.5 at 25 °C.

Physical form

Solution in 50% glycerol, 5 mM potassium phosphate, pH 6.0

Analysis Note

Protein determined by biuret.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Adenosine reagent-free detection by co-immobilization of adenosine deaminase and phenol red on an optical biostrip
Bartzoka F, et al.
Biotechnology Journal, 10(1), 136-142 (2015)
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Yeon-Mi Lee et al.
Biophysical chemistry, 172, 18-25 (2013-01-22)
Human ADAR1, which has two left-handed Z-DNA binding domains, preferentially binds Z-DNA rather than B-DNA with a high binding affinity. Z-DNA can be induced in long genomic DNA by Z-DNA binding proteins through the formation of two B-Z junctions with
Leila Antonangelo et al.
Clinics (Sao Paulo, Brazil), 67(11), 1259-1263 (2012-11-28)
Pleural tuberculosis is the most frequently occurring form of extra pulmonary disease in adults. In up to 40% of cases, the lung parenchyma is concomitantly involved, which can have an epidemiological impact. This study aims to evaluate the pleural and
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Immune status of waste management workers (WMW) with underlying systemic inflammation was assessed to identify useful immune-related biomarkers of occupational health and safety. Clinical history of WMW revealed high prevalence of respiratory symptoms alongside gastrointestinal and musculoskeletal complaints relative to

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