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A5353

Sigma-Aldrich

Apolipoprotein B from human plasma

≥95%, lyophilized powder

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Synonym(s):
apoB
MDL number:
NACRES:
NA.25

biological source

human plasma

Quality Level

Assay

≥95%

form

lyophilized powder

UniProt accession no.

shipped in

ambient

storage temp.

−20°C

Gene Information

human ... APOB(338)

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This Item
ALP30A0972A3106
assay

≥95%

assay

>95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-GE)

form

lyophilized powder

form

solid

form

buffered aqueous solution

form

buffered aqueous solution

shipped in

ambient

shipped in

dry ice

shipped in

dry ice

shipped in

-

storage temp.

−20°C

storage temp.

-

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

Biochem/physiol Actions

Apo-B levels correlate with the risk of coronary disease. Apo-B exists in human plasma in two isoforms, Apo-B48 and Apo-B100. Apo-B100 is the major physiological ligand for the LDL receptor. Apo-B100 is a large monomeric protein, containing 4563 amino acids (MW 515 kDa). Apo-B100 is synthesized in the liver and is required for the assembly of VLDL. It does not interchange between lipoprotein particles, as do the other apolipoproteins, and it is found in IDL and LDL after the removal of the Apo-A, E, and C. Apo-B48 is present in chylomicrons and their remnants. It is essential for the intestinal absorption of dietary lipids.
Apo-B48 is synthesized in the small intestine. It comprises approximately half of the N-terminal region of Apo-B100 and is the result of posttranscriptional mRNA editing by a stop codon in the intestine not found in the liver. The concentration of Apo-B in normal plasma is ~90 mg/dl.

Physical form

Lyophilized from buffer containing 10 mM sodium deoxycholate, 0.05 M sodium carbonate and 0.05 M sodium chloride, pH 10.0

Preparation Note

Delipidated with sodium deoxycholate.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Eye Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


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Moon Kyung Choe et al.
Pharmaceuticals (Basel, Switzerland), 14(4) (2021-05-01)
Obesity is associated with autoimmunity, a phenomenon considered as harmful. Here we show that obese mice and humans produce IgG-type autoantibodies that specifically recognize apolipoprotein B-100 (ApoB100), its native epitope p210, and the synthetic p210 mimotope pB1. By contrast, antibodies

Articles

Since cholesterol is a water-insoluble molecule it must be packaged for transport within the plasma. The particles that package cholesterol, cholesteryl esters, and triglycerides for transport, are called lipoproteins.

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