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A7005

Sigma-Aldrich

β-Amylase from sweet potato

greener alternative

Type I-B, ammonium sulfate suspension, ≥750 units/mg protein (E1%/280)

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Synonym(s):
1,4-α-D-Glucan maltohydrolase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.54

biological source

sweet potato

Quality Level

type

Type I-B

form

ammonium sulfate suspension

specific activity

≥750 units/mg protein (E1%/280)

greener alternative product score

old score: 57
new score: 1
Find out more about DOZN™ Scoring

greener alternative product characteristics

Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

concentration

≥7.0 mg/mL

greener alternative category

storage temp.

2-8°C

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This Item
A7130A8220A9857
β-Amylase from barley Type II-B, 20-80 units/mg protein (biuret)

Sigma-Aldrich

A7130

β-Amylase from barley

form

ammonium sulfate suspension

form

powder

form

aqueous solution

form

powder

specific activity

≥750 units/mg protein (E1%/280)

specific activity

20-80 units/mg protein (biuret)

specific activity

≥800 FAU/g

specific activity

≥150 units/mg protein (biuret)

concentration

≥7.0 mg/mL

concentration

-

concentration

-

concentration

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

β-Amylases belongs to the class of amylolytic enzymes.
We are committed to bringing you Greener Alternative Products, which adhere to one of the four categories of Greener Alternatives . This product belongs to category of Re-engineered products, showing key improvements in Green Chemistry Principles “Waste Prevention”, “Safer Solvents and Auxiliaries” and “Enhanced Energy Efficiency”. Click here to view its DOZN scorecard.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Application

β -Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. β -Amylase, from Sigma, has been used in various plant studies, such as carbon starvation studies in Populus tremuloides. β-amylase from sweet potato has been used to examine the utility of the enzyme in inhibiting and removing Staphylococcus aureus biofilms. The enzyme has also been used to prepare β-limit dextrin from waxy maize starch.
β-Amylase from sweet potato has been used:
  • in the hydrolysis of starch and starch-pectin combinations
  • for comparing amylases from different sources in Staphylococcus aureus SH1000 biofilm inhibition and reduction assay
  • for selenium speciation in samples

Biochem/physiol Actions

β-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. β-amylase from sweet potato is a tetramer of identical subunits, which are arranged to exhibit 222 molecular symmetry.
β-Amylases plays a major role in examining enzymatic promiscuity among glycosidases.

Unit Definition

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 4.8 at 20 °C.

Physical form

Crystalline suspension in 2.3 M (NH4)2SO4

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3


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Effect of cross-linking on the resistance to enzymatic hydrolysis of waxy maize starch and low-methoxy pectin
Khondkar D, et al.
Food Hydrocolloids, 23(2), 387-393 (2009)
Mechanisms of enzymatic glycosyl transfer
Comprehensive Natural Products II (2010)
Bradford Craigen et al.
The open microbiology journal, 5, 21-31 (2011-07-16)
Staphylococcus aureus, a versatile human pathogen, is commonly associated with medical device infections. Its capacity to establish and maintain these infections is thought to be related to its ability to form adherent biofilms. In this study, commercially available α-amylase compounds
Xin Qi et al.
Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques, 14(1), 60-66 (2011-04-20)
β-Limit dextrin has been studied for many years as a means to investigate the internal structures of amylose and amylopectin. However its role as an excipient in the pharmaceutical industry has never been reported. This paper is the first one
C G Cheong et al.
Proteins, 21(2), 105-117 (1995-02-01)
Sweet potato beta-amylase is a tetramer of identical subunits, which are arranged to exhibit 222 molecular symmetry. Its subunit consists of 498 amino acid residues (Mr 55,880). It has been crystallized at room temperature using polyethylene glycol 1500 as precipitant.

Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocols

Enzymatic Assay of β-AMYLASE (EC 3.2.1.2)

Beta-amylase is found in bacteria, fungi, and plants. To measure β-amylase activity, this assay uses a colorimetric spectrophotometric stop reaction at 540 nm.

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