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A7130

Sigma-Aldrich

β-Amylase from barley

greener alternative

Type II-B, 20-80 units/mg protein (biuret)

Synonym(s):

1,4-α-D-Glucan maltohydrolase

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About This Item

CAS Number:
9000-91-3
Enzyme Commission number:
3.2.1.2 (BRENDA, IUBMB)
EC Number:
232-566-1
MDL number:
MFCD00081391
UNSPSC Code:
12352204
NACRES:
NA.54

type

Type II-B

Quality Level

200

form

powder

specific activity

20-80 units/mg protein (biuret)

greener alternative product characteristics

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

greener alternative category

, Enabling

shipped in

wet ice

storage temp.

2-8°C

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General description

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Application

β -Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. β -Amylase, has been used in various plant studies, such as carbon starvation studies in Populus tremuloides . β -Amylase, from barley, has been used to study how pressure and temperature affect catalytic activity .

Biochem/physiol Actions

β-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. Pure, crystalline β-amylase preparation consists of four isoenzymes with different isoelectric points. The enzyme polymerizes very rapidly through the sulfhydryl groups in the absence of reducing agents. p-Chloromercuribenzoate inhibits the polymerization and the enzymatic activity. The reducing agents mercaptoethanol or dithiothreitol can completely restore the activity.

Quality

Crude

Unit Definition

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 4.8 at 20 °C.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificates of Analysis (COA)

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Volker Heinz et al.
Biotechnology progress, 21(6), 1632-1638 (2005-12-03)
The depolymerization of starch by beta-amylase during exposure to hydrostatic pressure up to 700 MPa and within a temperature range from 20 to 70 degrees C has been investigated. Inactivation of the enzyme as well as alterations in conversion speed
Purification and characterisation of crystalline -amylase from barley.
K Visuri et al.
European journal of biochemistry, 28(4), 555-565 (1972-08-04)
William R L Anderegg et al.
Plant physiology, 159(4), 1866-1874 (2012-06-06)
Drought impacts on forests, including widespread die-off, are likely to increase with future climate change, although the physiological responses of trees to lethal drought are poorly understood. In particular, in situ examinations of carbon starvation and its interactions with and
Claudinéia Aparecida Soares et al.
Journal of agricultural and food chemistry, 59(12), 6672-6681 (2011-05-20)
Different banana cultivars were used to investigate the influences of starch granule structure and hydrolases on degradation. The highest degrees of starch degradation were observed in dessert bananas during ripening. Scanning electron microscopy images revealed smooth granule surface in the
Heike Reinhold et al.
The Plant cell, 23(4), 1391-1403 (2011-04-14)
Plants contain β-amylase-like proteins (BAMs; enzymes usually associated with starch breakdown) present in the nucleus rather than targeted to the chloroplast. They possess BRASSINAZOLE RESISTANT1 (BZR1)-type DNA binding domains--also found in transcription factors mediating brassinosteroid (BR) responses. The two Arabidopsis
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Protocols

Enzymatic Assay of β-AMYLASE (EC 3.2.1.2)

Beta-amylase is found in bacteria, fungi, and plants. To measure β-amylase activity, this assay uses a colorimetric spectrophotometric stop reaction at 540 nm.

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