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A8094

Sigma-Aldrich

L-Arginine

from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 98.5-101.0%

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Synonym(s):
(S)-2-Amino-5-guanidinopentanoic acid
Linear Formula:
H2NC(=NH)NH(CH2)3CH(NH2)CO2H
CAS Number:
Molecular Weight:
174.20
Beilstein:
1725413
EC Number:
MDL number:
eCl@ss:
32160406
PubChem Substance ID:

biological source

non-animal source

Quality Level

Agency

USP/NF
meets EP testing specifications
meets USP testing specifications

product line

BioXtra

Assay

98.5-101.0%

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

color

white

mp

222 °C (dec.) (lit.)

solubility

H2O: 100 mg/mL

application(s)

agriculture
peptide synthesis
pharmaceutical (small molecule)

SMILES string

N[C@@H](CCCNC(N)=N)C(O)=O

InChI

1S/C6H14N4O2/c7-4(5(11)12)2-1-3-10-6(8)9/h4H,1-3,7H2,(H,11,12)(H4,8,9,10)/t4-/m0/s1

InChI key

ODKSFYDXXFIFQN-BYPYZUCNSA-N

Gene Information

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1 of 4

This Item
A5006W381918W381920
L-Arginine from non-animal source, meets EP, USP testing specifications, suitable for cell culture, 98.5-101.0%

Sigma-Aldrich

A8094

L-Arginine

Premium Grade
L-Arginine reagent grade, ≥98%

Sigma-Aldrich

A5006

L-Arginine

Essential Grade
L-Arginine 99%, FCC, FG

Sigma-Aldrich

W381918

L-Arginine

-
L-Arginine natural, FCC, FG

Sigma-Aldrich

W381920

L-Arginine

-
assay

98.5-101.0%

assay

≥98%

assay

99%

assay

-

form

powder

form

powder

form

powder or crystals

form

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

technique(s)

-

impurities

endotoxin, tested

impurities

-

impurities

-

impurities

-

color

white

color

white

color

-

color

-

Application

L-Arginine has been used:
  • as a Roswell park memorial institute medium (RPMI) media component in the isolation and culture of peripheral blood mononuclear cells (PBMCs)
  • as a RPMI media component for tissue culture
  • in DMEM medium for the identification and quantification of phosphorylation sites by stable isotope labeling by amino acids in cell culture (SILAC) and LCMS/MS

Biochem/physiol Actions

L-Arginine is a conditionally essential amino acid, that play a vital role in the production of nitric oxide. It acts as a precursor for the synthesis of polyamines, agmatine and urea. L-Arginine is usually essential in children and non-essential in adults.
Substrate of nitric oxide synthase, which is converted to citrulline and nitric oxide (NO). Induces insulin release by a nitric oxide-dependent mechanism.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Requirement of the mTOR Kinase for the Regulation of Maf1 Phosphorylation and Control of RNA Polymerase III-dependent Transcription in Cancer Cells
Boris Shor
The Journal of Biological Chemistry, 285(20), 15380-15392 (2010)
Association of reactive oxygen and nitrogen intermediate and complement levels with apoptosis of peripheral blood mononuclear cells in lupus patients
Oates J C, et al.
Arthritis and Rheumatism, 56(11), 3738-3747 (2007)
J. P. Felix D'Mello
Amino Acids in Human Nutrition and Health, 225-225 (2012)
Cell-specific Labeling Enzymes for Analysis of Cell?Cell Communication in Continuous Co-culture
Tape CJ, et.al.
Molecular and Cellular Proteomics, 13(7), 1866-1876 (2014)
Anti-aging effects of L-arginine
Gad M Z
Journal of Advanced Research, 1(3), 169-177 (2010)

Protocols

Perform colorimetric assays for nonradioactive quantification of cellular proliferation, viability, and cytotoxicity for adherent or suspension cells cultured in 96-well microplates.

MTT assay protocol for measuring cell viability, proliferation and cytotoxicity. Instructions for MTT reagent preparation and examples of applications.

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