A8312
m-Aminophenylboronic acid–Agarose
aqueous suspension
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Synonym(s):
(3-aminophenyl)boronic acid-Agarose, 3-Aminophenylboronic acid–Agarose, m-APBA-Agarose, m-Aminophenylboronic acid resin
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form
aqueous suspension
Quality Level
extent of labeling
40-80 μmol per mL
matrix
6% beaded agarose
matrix activation
epichlorohydrin
matrix attachment
through amino to carboxyls of EDTA
matrix spacer
9 atoms
capacity
≥8 mg/mL binding capacity (peroxidase Type VI)
storage temp.
2-8°C
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General description
m-Aminophenylboronic acid (m-APBA) immobilized on an agarose gel is useful in immunoglobulins capture, but also leads to non-specific interactions. m-APBA is a boronate affinity matrix that is effectively used for affinity purification of monoclonal antibodies.
Application
m-Aminophenylboronic acid-Agarose has been used:
- as a component of the column for the purification of Escherichia coli TOP10F cells
- with cell lysates of various cells for detection of PARylated (Poly(ADP-ribos)ylation) proteins.
- in the affinity purification of horseradish peroxidase (HRPO)
- in the glycated human serum albumin (gHSA) purification
Physical form
Suspension in water containing 0.002% chlorhexidine diacetate.
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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