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A8781

Sigma-Aldrich

β-Amylase from sweet potato

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Synonym(s):
1,4-α-D-Glucan maltohydrolase
CAS Number:
Enzyme Commission number:
EC Number:
MDL number:
NACRES:
NA.56

form

powder

Quality Level

mol wt

~200,000

packaging

vial of 15 mg

greener alternative product score

old score: 57
new score: 1
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greener alternative product characteristics

Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
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storage temp.

−20°C

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1 of 4

This Item
A7005A9857A6237
vibrant-m

A8781

β-Amylase from sweet potato

vibrant-m

A7005

β-Amylase from sweet potato

vibrant-m

A9857

α-Amylase from Aspergillus oryzae

vibrant-m

A6237

Apyrase from potato

mol wt

~200,000

mol wt

-

mol wt

-

mol wt

-

Quality Level

100

Quality Level

200

Quality Level

200

Quality Level

200

form

powder

form

ammonium sulfate suspension

form

powder

form

lyophilized powder

packaging

vial of 15 mg

packaging

-

packaging

-

packaging

-

greener alternative product score

old score: 57
new score: 1
Find out more about DOZN™ Scoring

greener alternative product score

old score: 57
new score: 1
Find out more about DOZN™ Scoring

greener alternative product score

-

greener alternative product score

-

General description

β-Amylase from sweet potato is a tetrameric enzyme with each monomer comprising (α/β) core domain and a C-terminal loop region. It belongs to the glycoside hydrolase family GH14.
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Application

β-Amylase from sweet potato has been used:
  • as a molecular weight marker in blue native polyacrylamide gel electrophoresis (PAGE)
  • as a standard for calibration curve preparation in size exclusion chromatography
  • as a model protein for small-angle X-ray analysis (SAXs)

Biochem/physiol Actions

β-Amylase acts on the polysaccharide α(1,4) glycosidic linkages and releases maltose present in the non-reducing ends. Due to its stability, β-Amylase from sweet potato is used in the immobilized enzyme preparation in combination with agarose beads.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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BMC structural biology, 16(1), 18-18 (2016-10-30)
Synchrotron radiation facilities are pillars of modern structural biology. Small-Angle X-ray scattering performed at synchrotron sources is often used to characterize the shape of biological macromolecules. A major challenge with high-energy X-ray beam on such macromolecules is the perturbation of
Khalilah G Reddie et al.
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Integration of affinity precipitation with partitioning methods for bioseparation of chitin binding lectins.
Teotia, S. et al.
Food and Bioproducts Processing, 84, 37-43 (2006)
Optimization of the immobilization of sweet potato amylase using glutaraldehyde-agarose support. Characterization of the immobilized enzyme
Tavano OL, et al.
Process. Biochem., 48(7), 1054-1058 (2013)
Liyu Tu et al.
Journal of cell science, 119(Pt 24), 5077-5086 (2006-12-13)
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Protocols

Gel filtration chromatography is an established method for determining the size and molecular mass of proteins.

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