A9647
Bovine Serum Albumin
heat shock fraction, pH 7, ≥98%
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
About This Item
Recommended Products
biological source
bovine
Quality Level
assay
≥98%
form
lyophilized powder
mol wt
~66 kDa
purified by
heat shock fractionation
origin
USA origin
technique(s)
immunocytochemistry: suitable
impurities
≤5.0% Loss on drying
loss
≤5%
pH
7
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
BT virus, none detected
VSV virus, none detected
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
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General description
Application
- BSA has been used in cell culture, e.g. as a supplement in media prepared for culturing of oligodendrocytes.
- BSA has been used for the dilution of primary antibody solution in immunohistochemistry procedures.
- BSA has been used as a standard in the Bradford method of protein quantification.
- BSA has been used in blocking and permeabilization buffer for immunofluorescence studies.
Biochem/physiol Actions
Preparation Note
also commonly purchased with this product
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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Articles
This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.
Experience effective immobilization of protein A on wide-pore epoxy-modified silica monoliths for mAb separation, showcasing the efficiency of Chromolith® WP 300 columns in achieving faster and highly reproducible biomolecule separations.
Explore serum albumin's role in serum-free cell culture systems for biomanufacturing.
Protocols
To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
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