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A9731

Sigma-Aldrich

Albumin human

recombinant, expressed in rice, lyophilized powder, suitable for cell culture, low endotoxin, ≥96% (SDS-PAGE)

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Synonym(s):
Cellastim S, rHSA, rHSA
CAS Number:
MDL number:
eCl@ss:
42010201
NACRES:
NA.25

biological source

human

Quality Level

recombinant

expressed in rice

Assay

≥96% (SDS-PAGE)

form

lyophilized powder

mol wt

monomer ~67 kDa

technique(s)

cell culture | mammalian: suitable

impurities

≤1 EU/mg protein

UniProt accession no.

storage temp.

2-8°C

InChI

1S/C3F8/c4-1(5,2(6,7)8)3(9,10)11

InChI key

QYSGYZVSCZSLHT-UHFFFAOYSA-N

Gene Information

human ... ALB(213)

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1 of 4

This Item
A7223A1887A4327
Albumin human recombinant, expressed in rice, lyophilized powder, suitable for cell culture, low endotoxin, ≥96% (SDS-PAGE)

Sigma-Aldrich

A9731

Albumin human

Albumin human recombinant, expressed in Pichia pastoris, 5% in aqueous buffer, ≥90% (SDS-PAGE)

Sigma-Aldrich

A7223

Albumin human

assay

≥96% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

assay

≥96% (agarose gel electrophoresis)

assay

≥96% (agarose gel electrophoresis)

form

lyophilized powder

form

solution

form

lyophilized powder

form

lyophilized powder

mol wt

monomer ~67 kDa

mol wt

monomer ~67 kDa

mol wt

monomer calculated mol wt 66478 Da

mol wt

monomer calculated mol wt 66478 Da

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

ELISA: suitable, tissue culture: suitable, western blot: suitable

technique(s)

tissue culture: suitable

impurities

≤1 EU/mg protein

impurities

-

impurities

HIV I and HIVII, HCV and HBsAg, tested negative, ≤0.007% Fatty Acids

impurities

HIV I and HIVII, HCV and HBsAg, tested negative

Application

This recombinant variant is recommended for use in absorption, distribution, metabolism, and excretion (ADME) pharmacological research; cell culture; drug delivery research; and cryopreservation of cells.

Biochem/physiol Actions

Albumins are soluble monomeric proteins found in the body fluids and tissues of animals and in some plant seeds. Serum albumin functions as a carrier protein for steroids, fatty acids, and thyroid hormones. Serum albumins are also vital in regulating the colloidal osmotic pressures of blood.

Features and Benefits

Cellastim is a well defined, animal-free recombinant human albumin (rHA). Designed for cell culture applications, Cellastim is a powerful media supplement that outperforms plasma-derived human albumin, bovine serum albumin, and other sources of recombinant human albumin. Cellastim delivers consistent performance, not the variability associated with hydrolysates and extracts. Cellastim is ideally suited for use in cell culture media for Chinese Hamster Ovary (CHO), hybridoma, VERO, stem cells and primary cells.

Advantages
  • Eliminates animal-derived additives
  • Safe and regulatory friendly
  • Proven effective in enhancing stem cell growth in embryonic, mesenchymal, neural, cardiac, liver, kidney, keratinocyte and fibroblast stem cell lines
  • Maintains stem cells in an undifferentiated state
  • Eliminates coating of culture plates for adherent cell lines
  • Provides well-defined and consistent stem cell medium

Analysis Note

Assayed by SDS-PAGE

Other Notes

1). Patent Rights
EP2230311
U.S. Patent No. 10,618,951
U.S. Patent No. 10,981,974
U.S. Patent No. 11,492,3892.
2). Registered Trademark Rights
U.S. Reg. 5,696,531
International Reg. 1,456,207
To view more information on Cellastim download the Cellastim Brochure, Cellastim CHO Poster, and Cellastim hybridoma Poster.
View more information on human serum albumin.

Legal Information

Cellastim is a trademark of InVitria

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Wireeya Chawjiraphan et al.
MethodsX, 7, 101114-101114 (2020-11-17)
A simple and sensitive graphene oxide-mediated fluorescence quenching aptasensor is developed to quantify albuminuria in urine samples. The developed aptasensor used the specific target binding property of aptamer and fluorescence quenching property of graphene oxide to determine the concentration of
D Moreno et al.
Theriogenology, 84(7), 1053-1060 (2015-08-19)
The aim of this study was to develop an in vitro embryo culture medium without either fetal calf serum or BSA, using various growth factors and cytokines (GFs-CYKs; IGF-I, IGF-II, bFGF, LIF, GM-CSF, TGF-β1, and PDGF-BB), and other molecules with
Yongjia Fan et al.
ACS biomaterials science & engineering, 3(8), 1510-1518 (2016-08-14)
The development of platforms for the expansion and directed differentiation of human pluripotent stem cells (hPSCs) in large quantities under xeno-free conditions is a key step toward the realization of envisioned stem cell-based therapies. Microcarrier bioreactors afford great surface-to-volume ratio
Yasuhiro Kawai et al.
PloS one, 13(5), e0196655-e0196655 (2018-05-26)
Serum albumin is the major intravascular antioxidant. Though oxidative stress plays an important role in the pathophysiology of Immunoglobulin A nephropathy (IgAN), the association between serum albumin and the progression of IgAN is not entirely understood. This retrospective cohort study
Malte Tiburcy et al.
STAR protocols, 1(1), 100032-100032 (2020-10-29)
This protocol describes a robust method for the generation of engineered human myocardium (EHM) from pluripotent stem cells (PSCs) in a multi-well plate under defined, serum-free conditions. By parallel culture of up to 48 EHM in one plate, contractile heart

Articles

This page segues to comprehensive insights on how serum albumin and other important cell culture components affect the performance of serum-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies. The page introduces the in vitro chemistry and biochemistry of albumin. These insights may also be applied to ex vivo and tissue engineering applications.

Lipid Induced Insulin Resistance

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