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A9891

Sigma-Aldrich

7-Amino-4-methylcoumarin

Chromophore for substrates

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Synonym(s):
Coumarin 120
Empirical Formula (Hill Notation):
C10H9NO2
CAS Number:
Molecular Weight:
175.18
Beilstein/REAXYS Number:
142231
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.32

description

chromophore for enzyme substrates

assay

≥98% (HPLC)

form

powder

mp

223-226 °C (lit.)

solubility

acetone: 10 mg/mL, clear, colorless to yellow

fluorescence

λex 365 nm; λem 440 nm in ethanol(lit.)

storage temp.

2-8°C

SMILES string

CC1=CC(=O)Oc2cc(N)ccc12

InChI

1S/C10H9NO2/c1-6-4-10(12)13-9-5-7(11)2-3-8(6)9/h2-5H,11H2,1H3

Inchi Key

GLNDAGDHSLMOKX-UHFFFAOYSA-N

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This Item
A3401257370M5639
vibrant-m

A9891

7-Amino-4-methylcoumarin

vibrant-m

257370

7-Amino-4-methylcoumarin

assay

≥98% (HPLC)

assay

≥98% (TLC)

assay

99%

assay

≥98.00% (TLC)

solubility

acetone: 10 mg/mL, clear, colorless to yellow

solubility

ethanol: 20 mg/mL, clear, colorless to light yellow

solubility

-

solubility

water: pyridine, 1:1: 4.90-5.10 mg/mL, clear, colorless to light yellow

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

form

powder

form

powder

form

solid

form

powder

fluorescence

λex 365 nm; λem 440 nm in ethanol(lit.)

fluorescence

-

fluorescence

-

fluorescence

-

application

7-Amino-4-methylcoumarin has been used:
  • to determine cathepsin B like- activity
  • as a substrate for leucine aminopeptidase (LAP)
  • in chymotryptic assay
  • as a standard to detect the activation of caspase-3 during
  • sanguinarine-induced damage
  • protection by human DEAD-box DDX3

Used as matrix for analysis of monosulfated disaccharides and as a co-matrix with 6-aza-2-thiothymidine for sulfated neutral and sialylated tri-and tetrasaccharides.

Biochem/physiol Actions

7-Amino-4-methylcoumarin is a coumarin derivative, which serves as a fluorescence reference standard to screen protease inhibitors.

Preparation Note

Soluble in DMSO (10 mg/mL), DMF, and acetone (10 mg/mL).

Other Notes

Reagent for preparing new fluorogenic AMC-based substrates for cystine aminopeptidase (and other hydrolases); used as reference compound in the enzyme assay.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves


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Issues in Chemistry and General Chemical Research (2012)
Regulation of p21 expression for anti-apoptotic activity of DDX3 against sanguinarine-induced cell death on intrinsic pathway
Nguyen CN, et al.
Phytomedicine, 153096-153096 (2019)
Chymase uptake by cardiomyocytes results in myosin degradation in cardiac volume overload
Powell PC, et al.
Heliyon, 5(4), e01397-e01397 (2019)
Significant activities of extracellular enzymes from a brown tide in the coastal waters of Qinhuangdao, China
Ou L, et al.
Harmful Algae, 74, 1-9 (2018)
Masayasu Taki et al.
Journal of the American Chemical Society, 130(38), 12564-12565 (2008-09-03)
We described here a coumarin-based dual-excitation ratiometric probe for cadmium, CadMQ. This fluorescence sensor has high quantum yields of 0.59 and 0.70 in the metal-free and Cd2+-bound forms, respectively, and has a dissociation constant of 0.16 nM for Cd2+. CadMQ

Articles

Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.

Nitric oxide (NO) as a signal transporter in neurons, endothelial cells and in the immune system.

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