B3928
Blue-White Select™ Screening Reagent
for selection of recombinant bacterial clones
About This Item
Recommended Products
grade
for molecular biology
Quality Level
form
liquid
usage
sufficient for 125 dishes (90 mm)
technique(s)
nucleic acid detection: suitable
suitability
suitable for nucleic acid staining
shipped in
dry ice
storage temp.
−20°C
Related Categories
General description
Features and Benefits
- Intense color contrast for easy colony selection
- Convenient, ready-to-use solution
Components
40 mg/mL X-Gal
(prepared in DMSO)
Principle
The technique is based on vectors such as the pUC and the M13mp series that carry a fragment of the β-galactosidase gene encoding an a-fragment of β-galactosidase. Exploitation of these vectors requires use of a bacteria strain carrying the complementing gene fragment to allow the assembly of an active complex, resulting in the formation of blue colonies. Disruption of the ß-galactosidase gene by insertion of a DNA fragment into the vector′s multiple cloning site results in the loss of functional β-galactosidase activity; these colonies remain white, allowing for easy differentiation between lac+ and lac- colonies.
Legal Information
related product
wgk_germany
WGK 3
flash_point_f
188.6 °F
flash_point_c
87 °C
ppe
Eyeshields, Gloves, type ABEK (EN14387) respirator filter
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Protocols
Blue/white color selection streamlines vector insert differentiation, enhancing cloning efficiency for molecular biologists.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service