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B6185

Sigma-Aldrich

Bionic Buffer, 10X Concentrate

for Northern and Southern blotting, solution

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Synonym(s):
Sodium Borate Electrophoresis Buffer
NACRES:
NA.25

grade

for molecular biology

Quality Level

form

solution

concentration

10 ×

impurities

DNase, RNase and nickase, none detected

pH

8.0-8.5 (20-25 °C)

storage temp.

room temp

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This Item
B6429B493093290
Sigma-Aldrich

Sigma-Aldrich

93290

TRIS borate – EDTA buffer solution

Essential+ Grade
form

solution

form

solution

form

liquid

form

solution

concentration

10 ×

concentration

-

concentration

-

concentration

-

impurities

DNase, RNase and nickase, none detected

impurities

-

impurities

-

impurities

DNases, none detected, RNases, none detected, phosphatases, none detected, proteases, none detected

pH

8.0-8.5 (20-25 °C)

pH

-

pH

-

pH

8.6-9.0

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

General description

Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for higher voltage runs, extremely rapid running times, and high-resolution band separation.

Application

Suitable for:
  • Use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures
  • Use with TBA and TAE gels
  • Preparation of agarose gels

Features and Benefits

  • Faster run times
  • Sharper bands
  • Easy to dilute and use
  • Flexible use with a variety of gel types

Packaging

The 4 L and 10 L sizes are supplied in dispensers with a spigot.

Caution

Test electrophoresis chambers to determine highest voltage. Voltages beyond the recommended range for your apparatus may result in blown fuses or damage to your apparatus.

Legal Information

Bionic is a trademark of Sigma-Aldrich Co. LLC

Pictograms

Exclamation markHealth hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Irrit. 2 - Repr. 1B

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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705578-5MG-PW

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MMYOMAG-74K-13

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Protocols

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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