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B6404

Supelco

BCIP®/NBT solution, premixed

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NACRES:
NA.32

form

liquid

Quality Level

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B19117209111681451001
BCIP®/NBT solution, premixed

B6404

BCIP®/NBT solution, premixed

-
BCIP®/NBT Liquid Substrate System ready to use solution

B1911

BCIP®/NBT Liquid Substrate System

-
NBT-BCIP® solution BioReagent, suitable as substrate for alkaline phosphatase in dot blots

72091

NBT-BCIP® solution

Essential+ Grade
NBT/BCIP Stock Solution

11681451001

NBT/BCIP Stock Solution

-

Application

BCIP/NBT solution, premixed has been used:
  • as a substrate to develop dot-blots
  • as a substrate to reveal alkaline phosphatase in the immunohistochemical analysis
  • to detect specific immune complexes in western blot analysis

Other Notes

0.48 mM NBT, 0.56 mM BCIP, 10 mM Tris and 59.3 mM MgCl2, pH approx. 9.2

Substrates

Substrate for visualizing alkaline phosphatase conjugates in Western blotting.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Sandra Fernandes Arruda et al.
Nutrients, 12(5) (2020-05-28)
The Brazilian savanna fruit, tucum-do-cerrado (Bactrissetosa Mart.) reduces hepatic hepcidin levels. Therefore, we investigated the effect of tucum-do-cerrado on the TfR/HFE and/or BMP/HJV/SMAD and JAK/STAT pathways, in normal and excess iron conditions. Rats were treated with: control diet (CT); control
Anti-apoptotic effects of human granulocyte colony-stimulating factor (G-CSF) on retinal ganglion cells after optic nerve crush are PI3K/AKT-dependent
Tsai R K, et al.
Experimental Eye Research, 90(5), 537-545 (2010)
Neuroprotective effects of recombinant human granulocyte colony-stimulating factor (G-CSF) in neurodegeneration after optic nerve crush in rats
Tsai R K, et al.
Experimental Eye Research, 87(3), 242-250 (2008)
The major human structural IgE epitope of the Brazil nut allergen Ber e 1: a chimaeric and protein microarray approach
Alcocer M J C, et al.
Journal of Molecular Biology, 343(3), 759-769 (2004)
Dietary iron concentration may influence aging process by altering oxidative stress in tissues of adult rats
Arruda L F, et al.
PLoS ONE, 8(4), e61058-e61058 (2013)

Articles

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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