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BCA1

Sigma-Aldrich

Bicinchoninic Acid Kit for Protein Determination

for 200-1000 μg/ml protein

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Synonym(s):
BCA protein assay
NACRES:
NA.32

usage

sufficient for ~500 assays

storage temp.

2-8°C

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This Item
QPBCAB9643DUO82047
Supelco

Supelco

B9643

Bicinchoninic Acid solution

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

20-25°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

Application

Bicinchoninic Acid Kit has been used for the protein estimation in salivary proteome samples, human embryonic kidney 293 cells and luteal cells.

Features and Benefits

  • Simple, sensitive colorimetric assay for proteins
  • Two stable reagents are used for the working solution
  • Faster and easier than the Lowry protein assay
  • Compatible with many detergents both ionic and nonionic
  • Less variation between different proteins than the Bradford dye-binding assay
  • Adaptable for use in microwell plates

Principle

Proteins reduce alkaline Cu(II) to Cu(I) in a concentration-dependent manner. Bicinchoninic acid is a highly specific chromogenic reagent for Cu(I), forming a purple complex with an absorbance maximum at 562 nm. The absorbance is directly proportional to protein concentration. This is an alternative to the Folin-Ciocalteu reagent for protein determination.

Kit Components Only

Product No.
Description

  • Bicinchoninic Acid Solution 1 L

  • 4%(w/v) CuSO4 · 5H2O Solution 25 mL

  • Protein Standard Solution 5 x 1

Pictograms

CorrosionEnvironment

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1

Storage Class Code

8A - Combustible, corrosive hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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T1503
Product Number
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25G
Pack Size/Quantity

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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Related Content

A wide variety of products for traditional protein quantitation techniques such as BCA and Bradford. Also, products for alternative assays such as Lowry, Micro Pyrogallol and FluoroProfile are also available for total protein determination.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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