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C0858

Sigma-Aldrich

Cyclooxygenase 2 human

≥70% (SDS-PAGE), recombinant, expressed in insect cells, aqueous solution, ≥8000 units/mg protein

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Synonym(s):
COX-2, Prostaglandin H synthase 2
MDL number:
NACRES:
NA.32

recombinant

expressed in insect cells Active recombinant N-terminal His-tagged COX-2 N580A mutant purified from insect cells.
expressed in insect cells

Quality Level

assay

≥70% (SDS-PAGE)

form

aqueous solution

specific activity

≥8000 units/mg protein

mol wt

dimer subunit mol wt 70-74 kDa

UniProt accession no.

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PTGS2(5743)

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C0733SAB2500267SRP0258
vibrant-m

C0858

Cyclooxygenase 2 human

vibrant-m

C0733

Cyclooxygenase 1 from sheep

vibrant-m

SRP0258

PDE1B Active human

assay

≥70% (SDS-PAGE)

assay

-

assay

-

assay

≥70% (SDS-PAGE)

specific activity

≥8000 units/mg protein

specific activity

≥1500 units/mg protein

specific activity

-

specific activity

≥2000 units/μg

form

aqueous solution

form

glycerol solution

form

buffered aqueous solution

form

aqueous solution

recombinant

expressed in insect cells Active recombinant N-terminal His-tagged COX-2 N580A mutant purified from insect cells.

recombinant

-

recombinant

-

recombinant

expressed in baculovirus infected insect cells

mol wt

dimer subunit mol wt 70-74 kDa

mol wt

dimer subunit mol wt 70 kDa

mol wt

-

mol wt

87 kDa

Biochem/physiol Actions

COX-2 catalyzes the conversion of arachidonic acid to prostaglandin H2 (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is the inducible form of cyclooxygenase, induced by phorbol esters, lipopolysaccharides, and cytokines. COX-2 is upregulated in the neovasculature of malignant tumors. Inhibition of COX-2 with nonsteroidal anti-inflammatory drugs (NSAIDs) is associated with decreased tumor growth.

Other Notes

Tagged with a six-residue histidine sequence near the N-terminus.

Unit Definition

One unit will consume 1.0 nanomole of oxygen per minute at pH 8 at 37 °C.

Physical form

Solution in 80 mM Tris, pH 8.0, with 0.3 mM DDC, 0.01% TWEEN® 20 and 10% glycerol

Substrates

KM for arachidonate = 6.5 μM. Exhibits activity and sensitivity to NSAIDs similar to the non-tagged enzyme.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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