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C-41200

Sigma-Aldrich

DetachKit

3 x 30 ml

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.71
Pricing and availability is not currently available.

packaging

pkg of 3 × 30 ml

technique(s)

cell culture | mammalian: suitable

shipped in

cooler

storage temp.

−20°C

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application(s)

pharmaceutical small molecule

application(s)

pharmaceutical

application(s)

pharmaceutical small molecule

application(s)

pharmaceutical

agency

traceable to USP 1612517

agency

traceable to USP 1612528

agency

traceable to BP 833, traceable to Ph. Eur. Y0000828, traceable to USP 1312539

agency

traceable to USP 1611900

grade

certified reference material, pharmaceutical secondary standard

grade

certified reference material, pharmaceutical secondary standard

grade

certified reference material, pharmaceutical secondary standard

grade

certified reference material, pharmaceutical secondary standard

mol wt

(342.69)

mol wt

-

mol wt

-

mol wt

-

packaging

pkg of 50 MG

packaging

pkg of 50 mg

packaging

pkg of 200 mg

packaging

pkg of 500 mg

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-30°C

General description

DetachKit-30

Application

The DetachKit was developed for gentle and effective detachment of adherent primary human cells in culture. Each batch is tested on primary human cells. The DetachKit is optimized for primary human cells but can also be used for primary animal cells and cell lines.

Components

Each DetachKit consists of three components: HEPES BSS (HEPES buffered Balanced Salt Solution), Trypsin/EDTA Solution and TNS (Trypsin Neutralization Solution). The DetachKit comes with a Trypsin/EDTA ratio of 0.04%/0.03%. HEPES BSS contains 30 mM HEPES, D-Glucose, NaCl, KCl, Na-Phosphate and Phenol Red. TNS contains 0.05% Trypsin Inhibitor from soybean and 0.1% Bovine Serum Albumin.

Quality

All lots of PromoCell′s DetachKits are subjected to comprehensive quality control tests. Each lot is routinely tested for use with primary human cells, the absence of cytotoxicity, and physical parameters such as osmolality and pH level. Approved in-house lots are used as a reference. In addition, all lots have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Storage and Stability

Store at -20°C in the dark immediately after arrival. Refrigerate thawed solution at 4 to 8°C. If stored properly, the product is stable until the expiry date stated on the label. Thawed solutions can be stored at 4 to 8°C and used for up to 6 weeks.

Subculture Routine

Click here for more information.

Storage Class

10 - Combustible liquids

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