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C4482

Sigma-Aldrich

CelLytic Y Cell Lysis Reagent

For yeast cells

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Synonym(s):
Yeast cell lysis reagent
NACRES:
NA.56

Quality Level

form

solution

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This Item
C1990B7310C8740
vibrant-m

C4482

CelLytic Y Cell Lysis Reagent

vibrant-m

C1990

CelLytic Express

vibrant-m

B7310

CelLytic B Cell Lysis Reagent

vibrant-m

C8740

CelLytic B Cell Lysis Reagent

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

CelLytic Y Cell Lysis Reagent is versatile, phosphate-free and non-denaturing. It provides an effective method for cell lysis and protein solubilization. Target proteins maintain immunoreactivity or biological function. The protocol is brief and preformed at room temperature. No extreme conditions or glass beads are required. Use only 2.5-5 ml/gram yeast cells.

Application

CelLytic Y Cell Lysis Reagent has been used in lysis of yeast cells expressing DNA repair, Rad protein, heat shock protein (ssa1) and human interleukin 4 (hIL4).

Features and Benefits

  • Fast: Cell lysis in just 20 minutes
  • Efficient: Three times more efficient than glass beads
  • Flexible: Lysis is enhanced by the addition of reducing reagent (DTT)
  • Gentle: Non-denaturing - preserves biological activity

Legal Information

CelLytic is a trademark of Sigma-Aldrich Co. LLC

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Hazard Classifications

Aquatic Chronic 3 - Eye Irrit. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3


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Customers Also Viewed

The beta6beta7 region of the Hsp70 substrate-binding domain mediates heat-shock response and prion propagation
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Articles

Our yeast cell lysis reagents are versatile, phosphate-free, and non-denaturing. CelLytic Y Cell Lysis reagent provides an effective method for yeast cell lysis and protein solubilization. The CelLytic Y Yeast Enzymatic Lysis Kit provides a convenient method for a highly efficient spheroplast formation and protein extraction from Saccharomyces cerevisiae, Pichia Pastoris and Schizosaccharomyces pombe yeast strains.

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

Related Content

An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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